Ultrasound-Assisted Ionic Liquid Extraction, Macroporous Resin Purification, and Hypoglycemic Effects of Total...

In this study, total flavonoids from Polygonum perfoliatum L. (PPTF) were extracted using ionic liquid-assisted ultrasound and purified by macroporous resin. After optimization, the total flavonoid content increased from 33.9% to 71.2%. HPLC identified hyperoside, kaempferol-3-O-rutinoside, and quercetin. In vitro experiments showed that PPTF exhibited good free radical scavenging activity and inhibitory effects on α-glucosidase and α-amylase. Within the concentration range of 3.13-12.50 μg/mL, PPTF was non-toxic to HepG2 cells and improved glucose uptake, promoted glycogen synthesis, and reduced lipid accumulation in insulin-resistant cells in a dose-dependent manner. In a type 2 diabetic mouse model induced by high-fat diet combined with streptozotocin, intragastric administration of PPTF for four weeks significantly lowered fasting blood glucose in the high-dose group, improved oral glucose tolerance and insulin sensitivity, increased hepatic and muscle glycogen, normalized serum lipid profiles, and alleviated pathological damage in the liver and pancreas. PPTF exhibits favorable hypoglycemic and lipid-regulating effects in both cellular and animal models, demonstrating its potential for development as an adjunctive functional product for glycemic management.