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ACANTHAMOEBA SP.S-11 PHAGOCYTOTIC ACTIVITY ON MYCOBACTERIUM LEPRAE IN DIFFERENT NUTRIENT CONDITIONS

Sepling Paling1, Ratna Wahyuni2, Ni'matuzahroh1

  • 1Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.

African Journal of Infectious Diseases
|April 6, 2018
PubMed
Abstract

Insights

Non-nutrient media enhances Acanthamoeba phagocytic activity against Mycobacterium leprae. This finding suggests optimizing culture conditions for studying leprosy transmission dynamics.

Area of Science:

  • Microbiology
  • Environmental Science
  • Infectious Diseases

Background:

  • Mycobacterium leprae causes leprosy and can persist in the environment via microbial hosts.
  • Acanthamoeba, a Free-Living Amoeba (FLA), is a potential environmental host for M. leprae.
  • Nutrient availability influences Acanthamoeba's phagocytic capabilities.

Purpose of the Study:

  • To investigate the phagocytic activity of Acanthamoeba sp.S-11 towards Mycobacterium leprae.
  • To compare phagocytosis rates under non-nutrient and rich-nutrient media conditions.

Main Methods:

  • Culturing Acanthamoeba sp.S-11 and M. leprae on distinct nutrient media.
  • Isolating and amplifying M. leprae intracellular DNA using Real-Time PCR (Q-PCR).

Main Results:

  • Acanthamoeba exhibited higher phagocytic activity towards M. leprae when cultured on non-nutrient media.
  • Rich-nutrient media resulted in lower phagocytosis rates compared to non-nutrient media.

Conclusions:

  • Non-nutrient media is recommended for optimizing Acanthamoeba sp. phagocytic activity against M. leprae.
  • This approach may be crucial for understanding environmental M. leprae reservoirs and transmission.
Keywords:
Acanthamoeba spMycbacterium lepraeNutrientPhagocytosisReal Time PCR

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