Related Experiment Video

Updated: Feb 12, 2026

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle VLP-Based Vaccines Using a Capture Assay
05:15

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle VLP-Based Vaccines Using a Capture Assay

Published on: February 10, 2022

4.3K

Identification of Streptococcus mitis321A vaccine antigens based on reverse vaccinology

Qiao Zhang1, Kexiong Lin1, Changzheng Wang1

  • 1Institute of Respiratory Disease, Xinqiao Hospital of Third Military Medical University, Chongqing 400037, P.R. China.

Molecular Medicine Reports
|April 6, 2018
PubMed

Insights

This study sequenced the Streptococcus mitis 321A genome to find vaccine targets. Researchers identified potential surface proteins and virulence factors for developing a new S. mitis vaccine.

Area of Science:

  • Genomics
  • Vaccinology
  • Microbial Pathogenesis

Background:

  • Streptococcus mitis can become pathogenic.
  • Developing vaccines against pathogenic S. mitis requires identifying key antigen targets.

Purpose of the Study:

  • To identify potential antigen targets for an effective vaccine against pathogenic Streptococcus mitis 321A.
  • To analyze the genome of S. mitis 321A for vaccine candidate identification.

Main Methods:

  • Whole-genome sequencing of S. mitis 321A using Illumina Hiseq2000.
  • Gene and tandem repeat prediction using Glimmer 3.02 and TRF 4.04.
  • Functional annotation via BLAST against KEGG and COG databases; phylogenetic analysis.

Main Results:

  • Genome assembly: 2,110,680 bp, 40.12% GC, 6 scaffolds, 9 contigs.
  • 1,944 genes predicted, including 119 TRFs, 56 microsatellites, 10 minisatellites, and 154 transposons.
  • Identified genes encoding surface proteins, secreted proteins, virulence factors, and essential genes crucial for vaccine development.

Conclusions:

  • The study provides genomic insights into S. mitis 321A's biological functions.
  • Identified putative antigen candidates for a potent S. mitis vaccine.
  • Genomic data supports the development of targeted S. mitis vaccines.

More Related Videos

Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development09:03

Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development

Published on: June 19, 2018

9.3K
In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model07:05

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model

Published on: November 28, 2017

10.4K

Related Concept Videos

Vaccinations01:51

Vaccinations

Overview
51.9K
Cancer Vaccines01:30

Cancer Vaccines

Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...
1.1K
Reversible and Irreversible Processes01:14

Reversible and Irreversible Processes

The thermodynamic processes can be classified into reversible and irreversible processes. The processes that can be restored to their initial state are called reversible processes. It is only possible if the process is in quasi-static equilibrium, i.e., it takes place in infinitesimally small steps, and the system remains at equilibrium However, these are ideal processes and do not occur naturally. An ideal system undergoing a reversible process is always in thermodynamic equilibrium within...
5.9K
Diode: Reverse bias01:14

Diode: Reverse bias

A diode is reverse-biased when the positive terminal of an external voltage source is connected to the n-type material and the negative terminal to the p-type material. This configuration opposes the natural direction of current flow through the diode, effectively increasing the width of the depletion region and the barrier potential. The reverse bias condition produces a minimal leakage current, primarily due to minority charge carriers. This leakage becomes significant when the reverse...
2.1K
Antigen Presenting Cells01:22

Antigen Presenting Cells

The immune system is a complex network of cells and molecules that protects the body from foreign invaders. T cells, a type of white blood cell, play a crucial role in this process. They recognize and attack foreign substances, such as pathogens, that enter the body.
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
3.4K
Modeling of Diode Reverse Characteristics01:14

Modeling of Diode Reverse Characteristics

In electronic circuits, reverse-biased diode configurations are critical for regulating voltage levels. Zener diodes exploit the reverse breakdown phenomenon and exhibit a controlled breakdown at a specific Zener voltage (VZ). They are designed to maintain a constant voltage across their terminals and are commonly used for voltage regulation in circuits.
When a reverse voltage applied to a Zener diode exceeds its breakdown voltage, the diode enters the breakdown region. At this point, the...
652