Related Experiment Video
Updated: Feb 12, 2026

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
Published on: June 3, 2018
Porous Magnetic Pseudo-Carbon Paste Electrode Electrochemical Biosensor for DNA Detection
Abstract:
A novel and sensitive electrochemical sensor based on magnetic porous pseudo-carbon paste electrode (MPPCPE) for DNA detection was described in this study. The MPPCPE was fabricated by mixing polymethyl methacrylate (PMMA) microspheres, as template, graphite powders, as filler, pyrrole, as precursor of polymer and magnetic powder. It was demonstrated that the fabricated MPPCPE had more sensitivity for detecting DNA when compared with magnetic carbon paste electrode (MPCPE). Results from anodic stripping voltammetry (ASV) experiments showed that the Ag peak current versus concentration of complementary DNA at MPPCPE had two linear regions. The first region demonstrated linearity over a concentration range from 0.5 to 20 nM, with correlation coefficient of 0.992, while the second linear region had concentration of complementary DNA at 0.005 to 0.1 nM range, with correlation coefficient of 0.957. The detection limit was as low as 0.005 nM and the electrochemical sensor was easy to preserve for a long time, and had good reusability and stability.
More Related Videos
Related Concept Videos
What is an Electrochemical Gradient?
The chemical gradient relies on differences in the abundance of a substance on the outside versus the inside of a cell and flows from areas of high to low ion concentration. In contrast, the electrical gradient revolves around an...
Porosity in Cement Paste
The balance of water to cement in the mix is...
The Carbon Cycle
Carbon Skeletons
Standard Electrode Potentials
DNA-only Transposons
The donor site from where the transposon is excised is either degraded or...

