Glutaraldehyde Cross-linking Modification of Decellularized Rat Kidney Scaffolds

The decellularized (DC) scaffolds retain three-dimensional structures for the stimulation of cell growth, with components of the extracellular matrix (ECM) relatively conserved between species. The strategy based on decellularized scaffolds provides a new way for organ regeneration, with a number of prominent advances having been reported in the past few years. While their lack of biomechanical strength and excessive degradation limit the clinical applications, therefore it is urgent to modify the DC scaffolds to improve the performance.In this article we describe a simple and robust modification protocol for DC rat kidney scaffolds. To modify, we perfuse DC rat kidneys with glutaraldehyde through the perfusion circulation of the decellularization. After cross-linking, kidney scaffolds are harvested for evaluation of histology, structural stability, and biocompatibility, involving water absorption testing, biomechanical testing, scanning electron microscopy, and several different histological and immunofluorescent analyses.