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Updated: Feb 12, 2026

Modeling and Imaging 3-Dimensional Collective Cell Invasion

Modeling and Imaging 3-Dimensional Collective Cell Invasion

Published on: December 7, 2011

Probing three-dimensional collective cancer invasion with DIGME

Amani A Alobaidi¹, Bo Sun¹

¹Department of Physics, Oregon State University, Weniger Hall, Corvallis, OR USA.

Cancer Convergence

|April 7, 2018

Abstract

Background:

Multicellular pattern formation plays an important role in developmental biology, cancer metastasis and wound healing. While many physical factors have been shown to regulate these multicellular processes, the role of ECM micro-to-meso scale geometry has been poorly understood in 3D collective cancer invasion.

Results:

We have developed a mechanical-based strategy, Diskoid In Geometrically Micropatterned ECM (DIGME). DIGME allows easy engineering of the shape of 3D tissue organoid, the mesoscale ECM heterogeneity, and the fiber alignment of collagen-based ECM all at the same time. We have employed DIGME to study the 3D invasion of MDA-MB-231 diskoids in engineered collagen matrix. We find that the collective cancer invasion is closely regulated by the micro-to-meso scale geometry of the ECM.

Conclusions:

We conclude that DIGME provides a simple yet powerful tool to probe 3D dynamics of tissue organoids in physically patterned microenvironments.

Keywords:

Collective migration ECM Tumor organoid

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