Related Experiment Video
Updated: Feb 12, 2026
Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
Structural characterization and antitumor activity of a polysaccharide from ramulus mori
Yajie Chen1, Xue Jiang1, Hongqing Xie1
1College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, China.
Abstract:
This study investigated the chemical characterization and antitumor effects of a polysaccharide from ramulus mori. A water-soluble polysaccharide, RMP1 with the estimated molecular weight of 137 kDa, was isolated and purified from ramulus mori through gel permeation chromatography. RMP1 is mainly composed of arabinose, xylose, glucose, galactose and rhamnose in a ratio of 0.56:0.37:0.17:1.00:0.08. Methylation and NMR analysis revealed that RMP1 had a backbone composed of 1,6-β-d-Galp, 1,3,6-β-d-Galp and 1,3-β-d-Galp residues, two main branches of 1,2-α-l-Araf, 1,3,6-β-d-Galp and 1,4-β-d-Xylp; it also had α-l-Araf and β-d-Glcp as terminals. In the MTT assay, RMP1 showed significant anticancer effects against the SGC-7901 and HeLa cells. In addition, no cytotoxicity was observed on the HEK-293 and RAW 264.7 cells. Flow cytometry showed that RMP1 exerted a stimulatory effect on the SGC-7901 cells apoptosis and induced the cell cycle arrest at the S phases. These findings suggest that RMP1 may serve as a potential novel antitumor agent.
Insights
A novel polysaccharide from Ramulus Mori, RMP1, demonstrates significant antitumor effects against SGC-7901 and HeLa cancer cells. This compound shows potential as a new antitumor agent without observed cytotoxicity in normal cells.
Area of Science:
- Natural Product Chemistry
- Pharmacology
- Cancer Research
Background:
- Ramulus Mori (Mulberry twig) is a traditional medicinal resource.
- Polysaccharides are known for diverse biological activities, including antitumor properties.
Purpose of the Study:
- To chemically characterize a polysaccharide from Ramulus Mori (RMP1).
- To investigate the antitumor effects and mechanism of action of RMP1.
- To evaluate the safety profile of RMP1.
Main Methods:
- Isolation and purification of RMP1 using gel permeation chromatography.
- Chemical characterization using methylation and Nuclear Magnetic Resonance (NMR) analysis.
- Antitumor activity assessed via MTT assay, with apoptosis and cell cycle analysis using flow cytometry.
Main Results:
- RMP1, a water-soluble polysaccharide (137 kDa), was isolated and characterized.
- RMP1 exhibited significant anticancer effects against SGC-7901 and HeLa cells.
- No cytotoxicity was observed in HEK-293 and RAW 264.7 cells; RMP1 induced apoptosis and S-phase cell cycle arrest in SGC-7901 cells.
Conclusions:
- RMP1 possesses a complex structure with potential therapeutic applications.
- RMP1 demonstrates selective antitumor activity and induces cancer cell death through apoptosis and cell cycle arrest.
- RMP1 represents a promising candidate for novel antitumor agent development.
Related Concept Videos
01:26Biosynthesis of Polysaccharides
01:15Cellulose and Pectic Polysaccharides
As a cell matures, its cell wall specializes according to its type. For example, the...
01:28Structure-Activity Relationships and Drug Design
SAR studies the intricate relationship between a drug's chemical structure and biological activity. It focuses on understanding how modifications to a drug's structure can influence...
01:29Cholinergic Antagonists: Chemistry and Structure-Activity Relationship
01:16Adrenergic Agonists: Chemistry and Structure-Activity Relationship
Aromatic ring substitutions: Substituting the aromatic ring with –OH groups at positions 3 and 4 yields catecholamines (e.g., epinephrine), which have a high affinity for adrenoceptors. Hydrogen bonding between –OH groups and receptors enhances adrenergic activity.
Separation of...
01:30Local Anesthetics: Chemistry and Structure-Activity Relationship