Enzyme Deposition by Polydimethylsiloxane Stamping for Biosensor Fabrication

Area of Science:

• Electrochemistry
• Biosensor Technology
• Materials Science

Background:

• Development of sensitive and selective biosensors is crucial for various applications.
• Enzyme immobilization is a key step in biosensor fabrication.
• Existing methods for enzyme deposition can be inefficient or lack precision.

Purpose of the Study:

• To develop a high-performance glucose biosensor using an efficient enzyme transfer method.
• To investigate the use of polydimethylsiloxane (PDMS) stamping for enzyme immobilization.
• To characterize the performance of the fabricated glucose biosensor.

Main Methods:

• Fabrication of biosensors on Pt electrode surfaces using polypyrrole and Nafion permselective films.
• Electrochemical deposition of a chitosan film for enzyme immobilization.
• Enzyme transfer using PDMS stamping with glucose oxidase (GOx) and subsequent crosslinking.

Main Results:

• The optimized glucose biosensor exhibited a linear range up to 2 mM.
• High sensitivity (29.4 ± 1.3 μA mM^-1 cm^-2) and a low detection limit (4.3 ± 1.7 μM) were achieved.
• Rapid response time (~2 s) and good selectivity were demonstrated.

Conclusions:

• PDMS stamping is an effective method for enzyme deposition in electroenzymatic biosensor fabrication.
• The developed glucose biosensor offers excellent performance characteristics.
• This technique is promising for applications requiring precise enzyme deposition, such as in neuroscience research.