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Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set
Published on: December 8, 2009
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Reprogramming p53-Deficient Germline Stem Cells Into Pluripotent State by Nanog
Yanmin Feng1,2, Yan Ning1,2, Xiwen Lin1
11 State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology , Chinese Academy of Sciences, Beijing, China .
Stem Cells and Development
|April 11, 2018
Summary
Spermatogonial stem cells (SSCs) can be reprogrammed into pluripotent stem cells. Adding Nanog and Tet1 improved reprogramming efficiency, with Nanog alone succeeding in p53-deficient cells.
Area of Science:
- Stem cell biology
- Reproductive biology
- Epigenetics
Background:
- Cultured mouse spermatogonial stem cells (SSCs), or germline stem cells (GSCs), can regain pluripotency spontaneously or through genetic modification.
- Current reprogramming methods face low efficiencies and poorly understood mechanisms.
Purpose of the Study:
- To investigate the mechanisms underlying GSC reprogramming to pluripotency.
- To identify factors that enhance GSC reprogramming efficiency.
Main Methods:
- Transcriptomic analysis of GSCs and embryonic stem cells.
- Reprogramming attempts using Yamanaka factors, with modifications.
- Assessment of in vitro and in vivo differentiation potential of derived cells.
Main Results:
- Differential expression of transcription factors and epigenetic modifiers between GSCs and ESCs was observed.
- Standard Yamanaka factors failed to reprogram GSCs, but inclusion of Nanog and Tet1 was successful.
- Nanog alone reprogrammed p53-deficient GSCs with 0.02‰ efficiency.
- GSC-derived cells showed differentiation capacity but had impaired chimera formation, possibly due to abnormal methylation.
Conclusions:
- GSCs can be reprogrammed to a pluripotent state through various pathways.
- Nanog and Tet1 play crucial roles in GSC reprogramming.
- Further research is needed to address methylation abnormalities in GSC-derived pluripotent cells.
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