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Updated: Feb 12, 2026

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line
Published on: February 17, 2016
Tropomyosin isoforms have specific effects on the transcriptome of undifferentiated and differentiated B35
Holly Stefen1, Alexandra Kalyna Suchowerska1, Bei Jun Chen2
1Neurodegenerative and Repair Unit School of Medical Science UNSW Sydney NSW Australia.
Abstract:
Tropomyosins, a family of actin-associated proteins, bestow actin filaments with distinct biochemical and physical properties which are important for determining cell shape and regulating many cellular processes in eukaryotic cells. Here, we used RNA-seq to investigate the effect of four tropomyosin isoforms on gene expression in undifferentiated and differentiated rat B35 neuroblastoma cells. In undifferentiated cells, overexpression of tropomyosin isoforms Tpm1.12, Tpm2.1, Tpm3.1, and Tpm4.2 differentially regulates a vast number of genes, clustering into several gene ontology terms. In differentiated cells, tropomyosin overexpression exerts a much weaker influence on overall gene expression. Our findings are particularly compelling because they demonstrate that tropomyosin-dependent changes are attenuated once the cells are induced to follow a defined path of differentiation.
Database:
Sequence data for public availability are deposited in the European Nucleotide Archive under the accession number PRJEB24136.
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