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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
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A narrow open tubular column for high efficiency liquid chromatographic separation
Huang Chen1, Yu Yang1, Zhenzhen Qiao2
1Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma 73019, USA. Shaorong.liu@ou.edu.
The Analyst
|April 11, 2018
Summary
High-resolution liquid chromatography using narrow open tubular columns achieves over 10 million plates per meter in under 10 minutes. This fast separation technique shows promise for analyzing complex peptide samples and trace analysis.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- Open tubular liquid chromatography (OTLC) is a powerful separation technique.
- Achieving high efficiency and speed in OTLC is crucial for complex sample analysis.
Purpose of the Study:
- To investigate the performance of extremely narrow open tubular columns in liquid chromatography.
- To explore the potential for achieving ultra-high efficiency and fast separations.
- To demonstrate the applicability of this technique for complex peptide sample analysis.
Main Methods:
- Utilizing open tubular columns with an inner diameter of 2 μm.
- Performing both isocratic and gradient elutions.
- Coating the column with octadecylsilane.
Main Results:
- Achieved over 10 million plates per meter in less than 10 minutes.
- Operated under an elution pressure of approximately 20 bar.
- Observed a focusing effect contributing to efficiency enhancement.
- Successfully separated complex peptide samples.
Conclusions:
- Extremely narrow open tubular columns offer a significant advancement in liquid chromatography efficiency and speed.
- The observed focusing effect enhances separation performance.
- This technique is a promising tool for high-resolution, fast separation of complex samples, including peptide analysis and trace analysis.
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