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Fabrication and Operation of Microfluidic Hanging-Drop Networks
Patrick M Misun1, Axel K Birchler2, Moritz Lang3
1Bio Engineering Laboratory, Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland. patrick.misun@bsse.ethz.ch.
None:
The hanging-drop network (HDN) is a technology platform based on a completely open microfluidic network at the bottom of an inverted, surface-patterned substrate. The platform is predominantly used for the formation, culturing, and interaction of self-assembled spherical microtissues (spheroids) under precisely controlled flow conditions. Here, we describe design, fabrication, and operation of microfluidic hanging-drop networks.
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