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Updated: Feb 12, 2026

Porcine Model of Infrarenal Abdominal Aortic Aneurysm
Published on: November 21, 2019
Validated UHPLC-MS/MS method for quantification of doxycycline in abdominal aortic aneurysm patients
Raghunadha Reddy Seelam1, Michael Terrin2, Hazem E Hassan1
1Pharmacokinetics & Biopharmaceutics Lab (PBL), Department of Pharmaceutical Science, School of Pharmacy, University of Maryland, Baltimore, MD 21201, USA.
Aim:
There is a strong evidence that doxycycline can benefit abdominal aortic aneurysms patients because of its ability to inhibit matrix metalloproteinase enzymes. There is a need for a specific quantification method for doxycycline in these patients. We report herein the development and validation of a selective, specific, simple and rapid UHPLC-MS/MS method for doxycycline.
Results:
The assay was linear (r2 > 0.994) over a concentration range of 0.010-7.005 μg/ml. This validated method has good lower limit of quantification (LLOQ) (10 ng/ml), accuracies (89.1-103.4%), precision (≤7.0%) and extraction recoveries (95.8%). Doxycycline was stable during short- and long-term storage.
Conclusion:
The assay is reliable and has been successfully applied to serum samples obtained from the patients of N-TA3CT clinical study.
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