Related Experiment Video
Updated: Feb 12, 2026
15:29
Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
Published on: August 5, 2016
8.6K
MULTILOCUS SEQUENCE TYPING OF BRUCELLA ISOLATES FROM THAILAND
Summary
Multilocus sequence typing (MLST) revealed unexpected genetic diversity among Brucella isolates in Thailand. This genetic classification helps understand brucellosis transmission in animals and humans.
Area of Science:
- Veterinary Microbiology
- Epidemiology
- Genetics
Background:
- Brucellosis causes significant economic losses in Thailand due to animal abortions and infertility.
- Brucella species exhibit high DNA homology, necessitating advanced typing methods.
Purpose of the Study:
- To genetically characterize local Brucella isolates using multilocus sequence typing (MLST).
- To determine the genetic relatedness and diversity of Brucella strains in Thailand.
- To identify potential transmission routes of brucellosis.
Main Methods:
- Brucella isolates were obtained from vaginal secretions of cattle and goats, and blood cultures from infected individuals.
- Species identification was performed using multiplex PCR.
- Multilocus sequence typing (MLST) was conducted on nine house-keeping genes.
Main Results:
- MLST analysis of 36 isolates identified 78 novel allele types and 34 novel sequence types (STs), with two known ST8 isolates.
- Genetic diversity was found to be 3.6%, with polymorphic sites ranging from 2-6%.
- Isolates were classified into three species: B. melitensis, B. abortus, and B. suis, consistent with PCR results.
Conclusions:
- MLST revealed significant and unexpected genetic diversity among Thai Brucella isolates.
- The study identified founder STs for B. abortus (ST2) and B. melitensis (ST8).
- Genetic classification provides insights into brucellosis transmission dynamics and regional/global strain relationships.
Related Concept Videos
01:23Types of RNA
73.0K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
73.0K
02:02Cis-regulatory Sequences
11.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.9K
01:29Sequences
295
Sequences are fundamental mathematical objects consisting of ordered lists of numbers that follow a specific rule or pattern. Sequences are critical in various mathematical concepts, including calculus, series, and number theory. They can model real-world phenomena such as population growth, financial investments, and physical processes like the diminishing height of a bouncing ball.Each number in a sequence is referred to as a term. Typically, the terms are denoted as a1, a2, a3,…, where...
295
01:57Sanger Sequencing
774.9K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
774.9K
01:30Arithmetic Sequences
243
An arithmetic sequence is a structured arrangement of numbers where each term is derived by adding a constant value, known as the common difference, to the previous term. This consistent pattern allows for the efficient computation of any term within the sequence as well as the cumulative sum of multiple terms. The formula for finding the nth term of an arithmetic sequence is:Here, aₙ represents the nth term of the sequence, a is the first term, d is the common difference, and n is the...
243
01:30Geometric Sequences
289
In systems where values diminish by a constant proportion at each stage, the resulting sequence follows a geometric structure. Each new value in the sequence is obtained by applying a fixed multiplier to the preceding term. This regular, proportional decline type is often used to represent processes involving gradual loss, such as energy dissipation or reduction in amplitude over time.When analyzing the total effect of such a process across unlimited iterations, the series of values is referred...
289