Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Networks02:26

Protein Networks

4.6K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.6K
Protein Networks02:26

Protein Networks

2.9K
2.9K
Network Covalent Solids02:18

Network Covalent Solids

16.2K
Network covalent solids contain a three-dimensional network of covalently bonded atoms as found in the crystal structures of nonmetals like diamond, graphite, silicon, and some covalent compounds, such as silicon dioxide (sand) and silicon carbide (carborundum, the abrasive on sandpaper). Many minerals have networks of covalent bonds.
To break or to melt a covalent network solid, covalent bonds must be broken. Because covalent bonds are relatively strong, covalent network solids are typically...
16.2K
Antibody Structure01:10

Antibody Structure

65.7K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
65.7K
Determining Order of Reaction02:53

Determining Order of Reaction

62.6K
Rate laws describe the relationship between the rate of a chemical reaction and the concentration of its reactants. In a rate law, the rate constant k and the reaction orders are determined experimentally by observing how the rate of reaction changes as the concentrations of the reactants are changed. A common experimental approach to the determination of rate laws is the method of initial rates. This method involves measuring reaction rates for multiple experimental trials carried out using...
62.6K
Structures of Solids02:22

Structures of Solids

18.2K
Solids in which the atoms, ions, or molecules are arranged in a definite repeating pattern are known as crystalline solids. Metals and ionic compounds typically form ordered, crystalline solids. A crystalline solid has a precise melting temperature because each atom or molecule of the same type is held in place with the same forces or energy. Amorphous solids or non-crystalline solids (or, sometimes, glasses) which lack an ordered internal structure and are randomly arranged. Substances that...
18.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Membrane Binding and Pro-Ferroptotic Activity of NQO1.

Chembiochem : a European journal of chemical biology·2026
Same author

Deficiency of N1-Adenine Methyltransferase Aggravates RNA and Protein Aggregation.

Cells·2025
Same author

The structural and functional roles of the flavin cofactor FAD in mammalian cryptochromes.

Frontiers in molecular biosciences·2023
Same author

Dynamic association of flavin cofactors to regulate flavoprotein function.

IUBMB life·2022
Same author

Mammalian Flavoproteome Analysis Using Label-Free Quantitative Mass Spectrometry.

Methods in molecular biology (Clifton, N.J.)·2021
Same author

Desmosome architecture derived from molecular dynamics simulations and cryo-electron tomography.

Proceedings of the National Academy of Sciences of the United States of America·2020
Same journal

Identification of Age-Associated Circulating Proteins and Lipids in 3800 Comorbidity-Enriched Older Adults from Japan-Based Cohorts Using Olink Assays and MRM Mass Spectrometry.

Journal of proteome research·2026
Same journal

Molecular Solution to the Paradox of Ancient Brain Preservation.

Journal of proteome research·2026
Same journal

From Method-Defined Signals to Reference Measurement Procedures: Two Decades of Mass Spectrometry-Based ProGRP Quantification.

Journal of proteome research·2026
Same journal

Proteomic Profiling of Extracellular Vesicle-Enriched Plasma Using Mag-Net for Biomarker Discovery in Pancreatic Ductal Adenocarcinoma.

Journal of proteome research·2026
Same journal

Computationally Efficient Bayesian Estimation of Graphical Networks for Omics Data.

Journal of proteome research·2026
Same journal

Hierarchy of MS-Based Evidence.

Journal of proteome research·2026
See all related articles

Related Experiment Video

Updated: Feb 12, 2026

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

30.6K

Polylysine is a Proteostasis Network-Engaging Structural Determinant.

Wei-Han Lang, Giulia Calloni, R Martin Vabulas

    Journal of Proteome Research
    |April 11, 2018
    PubMed
    Summary
    This summary is machine-generated.

    C-terminal polylysine (PL) triggers a cellular stress response. This study identifies key protein machinery in the cytosol that processes PL and reveals its novel association with nuclear transport, suggesting a new protein quality control pathway.

    Keywords:
    chaperonepolylysineproteasomeprotein degradationprotein quality controltranslational read-through

    More Related Videos

    Assessing the Multiple Dimensions of Engagement to Characterize Learning: A Neurophysiological Perspective
    13:57

    Assessing the Multiple Dimensions of Engagement to Characterize Learning: A Neurophysiological Perspective

    Published on: July 1, 2015

    13.2K
    Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors
    08:45

    Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors

    Published on: July 17, 2020

    6.6K

    Related Experiment Videos

    Last Updated: Feb 12, 2026

    Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
    22:00

    Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

    Published on: November 21, 2010

    30.6K
    Assessing the Multiple Dimensions of Engagement to Characterize Learning: A Neurophysiological Perspective
    13:57

    Assessing the Multiple Dimensions of Engagement to Characterize Learning: A Neurophysiological Perspective

    Published on: July 1, 2015

    13.2K
    Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors
    08:45

    Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors

    Published on: July 17, 2020

    6.6K

    Area of Science:

    • Cellular Biology
    • Protein Homeostasis
    • Molecular Mechanisms

    Background:

    • C-terminal polylysine (PL) synthesis occurs due to mRNA processing errors.
    • PL's positive charge causes ribosomal stalling, activating the Ribosome-associated quality control (RQC) complex.
    • RQC dysfunction leads to toxic PL-tagged protein accumulation and cellular stress.

    Purpose of the Study:

    • To investigate the specific protein quality control mechanisms for PL-tagged proteins.
    • To identify the cytosolic machinery involved in post-ribosomal PL processing.
    • To explore the interaction of PL with cellular transport systems.

    Main Methods:

    • Quantitative mass spectrometry to identify protein interactions.
    • Analysis of cytosolic protein complexes involved in proteostasis.
    • Investigating the role of nuclear transport machinery in PL handling.

    Main Results:

    • The study identified key cytosolic proteostasis network components, including TRiC chaperonins and proteasomes, involved in PL processing.
    • A novel association between PL-tagged polypeptides and the nuclear transport machinery was discovered.
    • Experimental evidence confirmed enhanced nuclear import of PL-tagged proteins, suggesting compartmentalization.

    Conclusions:

    • A previously unknown post-ribosomal PL-processing pathway exists in the human cytosol.
    • The nuclear transport machinery plays a role in handling PL-tagged proteins.
    • This suggests a novel cellular strategy for managing aberrant protein products.