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Enzyme pattern in endomyocardial biopsies from patients with chronic heart diseases
I Lehmann1, B Papies, P Romaniuk
1Inst. Pathol. Clin. Biochem., Humboldt-Univ. Berlin, GDR.
Insights
Biochemical markers like Lactate Dehydrogenase (LDH) and Aspartate Aminotransferase (ASAT) show characteristic changes in chronic heart disease. These alterations correlate with heart muscle cell hypertrophy and disease severity, aiding in patient assessment.
Area of Science:
- Cardiology
- Biochemistry
- Molecular Biology
Background:
- Chronic heart diseases encompass conditions like small vessel disease, cardiomyopathies, and hypertensive heart disease.
- Assessing the severity of these conditions often involves evaluating left ventricular end-diastolic pressure (LVEDP), ventricular kinetics, left ventricular heart mass (LVHM), and exercise electrocardiogram results.
Purpose of the Study:
- To investigate and compare isoenzymes of Lactate Dehydrogenase (LDH), Aspartate Aminotransferase (ASAT), and Glycogen Phosphorylase (GP) in patients with chronic heart disease.
- To determine if these biochemical markers correlate with the degree of cardiac hypertrophy and disease stage.
Main Methods:
- Endomyocardial biopsies were obtained from 67 patients diagnosed with various chronic heart diseases.
- Biopsies were analyzed for LDH and ASAT isoenzymes and GP.
- Results were compared to a reference group without evidence of chronic heart disease.
Main Results:
- Characteristic alterations in LDH and ASAT isoenzymes and GP were observed in patients with chronic heart disease.
- These biochemical changes were found to be dependent on the degree of heart muscle cell hypertrophy.
- The observed alterations also correlated with disease severity as indicated by LVEDP, ventricular kinetics, LVHM, and exercise ECG.
Conclusions:
- The study identified specific biochemical alterations in myocardial enzymes (LDH, ASAT) and Glycogen Phosphorylase (GP) in chronic heart disease.
- These biochemical markers reflect distinct metabolic states within the myocardium.
- The findings suggest that these biochemical alterations can serve as valuable supplementary information for assessing the severity of chronic heart disease.
Abstract:
In endomyocardial biopsies from 67 patients with various chronic heart diseases (small vessel disease, cardiomyopathies and hypertensive heart disease) the isoenzymes of Lactate Dehydrogenase (LDH) and Aspartate Aminotransferase (ASAT) and Glycogen Phosphorylase (GP) were investigated and compared with a reference group without actual morphological and functional evidence of chronic heart disease. The analyzed parameters showed characteristical alterations dependent on the degree of hypertrophy of the heart muscle cells and the stage of the disease as assessed by left ventricular enddiastolic pressure (LVEDP), ventricular kinetics, left ventricular heart mass (LVHM) and exercise electrocardiogram. The biochemical alterations found reflect different metabolic situations in the myocardium and could be useful as additional information for assessing the severity of the disease.