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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

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Related Experiment Video

Updated: May 8, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly.

B Ruskin1, P D Zamore, M R Green

  • 1Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.

Cell
|January 29, 1988
PubMed
Summary
This summary is machine-generated.

A newly identified factor, U2AF (U2 auxiliary factor), is essential for U2 snRNP binding to pre-mRNA. This discovery explains the requirement of the 3' splice site for initiating the splicing process.

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Related Experiment Videos

Last Updated: May 8, 2026

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Published on: August 9, 2019

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

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ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
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ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

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Area of Science:

  • Molecular Biology
  • RNA Splicing Mechanisms

Background:

  • Pre-mRNA splicing complex assembly involves specific interactions between U1 snRNP and the 5' splice site.
  • U2 snRNP binding to the branch point is also crucial for splicing complex formation.

Purpose of the Study:

  • To investigate the requirements for U2 snRNP interaction with pre-mRNA.
  • To identify factors necessary for U2 snRNP binding and splicing complex assembly.

Main Methods:

  • Purified U1 snRNP and partially purified U2 snRNP binding assays.
  • RNA substrate exclusion and competition assays to characterize U2AF activity.

Main Results:

  • Partially purified U2 snRNP alone could not bind to its target pre-mRNA sequence.
  • A novel activity, U2 auxiliary factor (U2AF), was identified as essential for U2 snRNP-branch point interaction.
  • U2AF binds to the 3' splice site region before U2 snRNP binds to the branch point.

Conclusions:

  • U2AF is a necessary component for U2 snRNP binding to the branch point.
  • The 3' splice site region's role in U2 snRNP binding is explained by U2AF mediation.
  • This finding elucidates a critical step in the initiation of pre-mRNA splicing.