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Updated: Feb 12, 2026

Intravitreous Injection for Establishing Ocular Diseases Model
Published on: October 1, 2007
Immunogenicity testing of therapeutic antibodies in ocular fluids after intravitreal injection
Uwe Wessels1, Markus Zadak1, Astrid Reiser1
1Pharmaceutical Sciences, Pharma Research & Early Development, Roche Innovation Center Munich, Nonnenwald 2, 82377 Penzberg, Germany.
Aim:
High drug concentrations in ocular fluids after intravitreal administration preclude the use of drug-sensitive immunoassays. A drug-tolerant immunoassay is therefore desirable for immunogenicity testing in ophthalmology.
Experimental:
Immune complex (IC) antidrug antibody (ADA) assays were established for two species. The assays were compared with the bridging assay in ocular and plasma samples from two preclinical studies.
Results:
The IC assays showed high drug tolerance, which enabled a reliable ADA detection in ocular fluids after intravitreal administration. The IC assays were superior to the bridging assay in the analysis of ocular fluids with high drug concentrations.
Conclusion:
The IC assay allows a reliable ADA detection in matrices with high drug concentrations, such as ocular fluids.
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