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The J gene of phi X174: isolation and characterization of a J gene mutant
R K Hamatake1, K J Buckley, M Hayashi
1Department of Chemistry, University of California, San Diego, La Jolla 92093.
Molecular & General Genetics : MGG
|January 1, 1988
Abstract:
The J gene protein of bacteriophage phi X174 is a component of the mature phage. The previous lack of J gene mutants has prevented an in vivo analysis of J protein functions. A phi X174 mutant was constructed by inserting an 11 nucleotide sequence into the J gene. This mutant, designated insJ, was viable only in the presence of a wild-type J gene carried on a plasmid that could provide J protein. An analysis of DNA synthesis during insJ mutant infection under non-permissive conditions confirmed that the J protein is not required for viral DNA synthesis.