Related Experiment Video

Updated: Feb 12, 2026

DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

21.0K

Replication-incompetent gammaretroviral and lentiviral vector-based insertional mutagenesis screens identify prostate

Victor M Bii1, Casey P Collins1, Jonah D Hocum1

  • 1College of Pharmacy, Washington State University, Spokane 99210, WA, USA.

Oncotarget
|April 13, 2018
PubMed

Insights

This study used gammaretroviral (γRV) and lentiviral (LV) vectors to identify genes driving prostate cancer progression. Complementary vector approaches revealed new candidate genes and potential biomarkers for predicting recurrence risk in patients.

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • Insertional mutagenesis screens using replication-incompetent gammaretroviral (γRV) and lentiviral (LV) vectors are established methods for identifying cancer driver genes.
  • These vectors integrate into the host genome, potentially dysregulating nearby genes and leading to cancer development.
  • γRV and LV vectors possess distinct integration profiles and genotoxic potentials, suggesting they can serve as complementary tools for mutagenesis screens.

Purpose of the Study:

  • To identify driver genes involved in the progression of androgen-independent prostate cancer (AIPC).
  • To leverage the complementary strengths of both γRV and LV vectors in insertional mutagenesis screens.
  • To discover potential biomarkers and therapeutic targets for prostate cancer.

Main Methods:

  • Utilized a xenotransplant mouse model with orthotopically injected, vector-transduced LNCaP cells.
  • Induced an androgen-deficient environment through castration to promote metastatic tumor development.
  • Employed a high-throughput modified genomic sequencing PCR (MGS-PCR) to identify vector integration sites in metastatic tumors.

Main Results:

  • Identified thirteen candidate prostate cancer progression genes: OR2A14, FER1L6, TAOK3, MAN1A2, MBNL2, SERBP1, PLEKHA2, SPTAN1, ADAMTS1, SLC30A5, ABCC1, SLC7A1, and SLC25A24.
  • Found that the expression of TAOK3 and ABCC1 in prostate cancer patients predicted the risk of recurrence following androgen deprivation therapy.
  • Demonstrated the complementary utility of γRV and LV vectors in uncovering cancer driver genes.

Conclusions:

  • Gammaretroviral (γRV) and lentiviral (LV) vectors are complementary tools for identifying cancer driver genes.
  • The identified candidate genes, particularly TAOK3 and ABCC1, show promise as potential biomarkers for prostate cancer recurrence.
  • These findings highlight novel therapeutic targets for prostate cancer progression.
Keywords:
Chromosomedriver genesgammaretroviral (γRV) vectorinsertional mutagenesis screenlentiviral (LV) vectorprostate cancer (PC)

More Related Videos

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells10:42

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells

Published on: December 12, 2017

16.3K
A Rapid Filter Insert-based 3D Culture System for Primary Prostate Cell Differentiation09:23

A Rapid Filter Insert-based 3D Culture System for Primary Prostate Cell Differentiation

Published on: February 13, 2017

8.9K

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
16.6K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.9K
Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
10.8K
Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
99.2K
DNA Replication02:40

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
60.2K
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
205.8K