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Mutagenesis Screens for Prostate Cancer Using Replication-Incompetent Lentiviral Vectors.

Grant D Trobridge1

  • 1Pharmaceutical Sciences, Washington State University, Spokane, WA, USA. grant.trobridge@wsu.edu.

Methods in Molecular Biology (Clifton, N.J.)
|December 14, 2018
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Summary

Researchers used lentiviral vectors (LVs) to mutagenize prostate cancer (PC) cells and identify genes driving progression to androgen-independent PC (AIPC). This method helps uncover key genetic factors in lethal AIPC development.

Keywords:
Androgen independenceLentiviral vectorMutagenesis screenOncogene discoveryProstate cancer

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Prostate cancer (PC) is a leading cause of cancer death in US men.
  • Progression to androgen-independent PC (AIPC) leads to metastasis and is often lethal.
  • Mechanisms driving PC progression to AIPC remain incompletely understood.

Purpose of the Study:

  • To describe protocols for generating replication-incompetent lentiviral vectors (LVs).
  • To outline a mutagenesis screen for identifying AIPC genes in vitro.
  • To identify novel genes involved in PC progression to AIPC.

Main Methods:

  • Utilized replication-incompetent lentiviral vectors (LVs) for mutagenesis.
  • Employed LVs as both mutagens and molecular tags.
  • Performed in vitro mutagenesis screens to identify candidate AIPC genes.

Main Results:

  • Successfully generated replication-incompetent LV preparations.
  • Established protocols for performing LV-based mutagenesis screens.
  • Identified candidate genes potentially involved in AIPC development through dysregulation by LV provirus.

Conclusions:

  • Lentiviral vector-mediated mutagenesis is a viable strategy for identifying AIPC genes.
  • This approach facilitates the discovery of novel genetic contributors to PC progression.
  • Further research can build upon these protocols to elucidate AIPC mechanisms.