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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
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Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
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Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes
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UXT is required for spermatogenesis in mice.

Eric D Schafler1,2, Phillip A Thomas1, Susan Ha1,3

  • 1Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY, United States of America.

Plos One
|April 13, 2018
PubMed
Summary
This summary is machine-generated.

Ubiquitous Expressed Transcript (UXT) is essential for male fertility. Its absence disrupts sperm production by impairing spermatogonial stem cell self-renewal and differentiation, leading to infertility.

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Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Genetics

Background:

  • Male mammals require continuous sperm production, relying on a balance between spermatogonial stem cell (SSC) self-renewal and germ cell differentiation.
  • Failures in this balance can lead to infertility.
  • The role of Ubiquitous Expressed Transcript (UXT) in mammalian spermatogenesis was previously undefined.

Purpose of the Study:

  • To investigate the in vivo function of Ubiquitous Expressed Transcript (UXT) in male mammalian germline development.
  • To elucidate the molecular mechanisms by which UXT influences spermatogenesis.

Main Methods:

  • Development of the first UXT knockout mouse model.
  • Conditional knockout of Uxt specifically in the male germline.
  • Histological analysis of testicular phenotypes.
  • Gene expression analysis to assess transcriptional changes.

Main Results:

  • Constitutive Uxt deletion resulted in embryonic lethality.
  • Conditional germline deletion of Uxt led to a Sertoli cell-only phenotype during the first wave of spermatogenesis, with no recovery in adults.
  • Uxt deletion downregulated genes critical for SSC self-renewal, differentiation, and meiosis.
  • Phenotype onset observed between 6-7 days postpartum, preceding meiotic entry.

Conclusions:

  • Ubiquitous Expressed Transcript (UXT) is indispensable for mammalian spermatogenesis.
  • UXT acts as a crucial regulator of distinct transcriptional programs governing SSCs and differentiating spermatogonia in vivo.
  • Disruption of UXT function leads to infertility due to impaired stem cell maintenance and germ cell development.