Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleic Acids02:43

Nucleic Acids

50.7K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
50.7K
Nucleic acids02:43

Nucleic acids

190.7K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
190.7K
Nucleic Acids02:43

Nucleic Acids

9.0K
9.0K
Nucleic Acid Structure01:25

Nucleic Acid Structure

9.4K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
9.4K
Nucleic Acids and Nucleotides01:20

Nucleic Acids and Nucleotides

15.2K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and have instructions for its functioning. The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
Deoxyribonucleic Acid (DNA)
DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and the organelles such as chloroplasts and mitochondria....
15.2K
Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

1.2K
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The chicken retrovirus-like gene <i>ENS-1/ERNI</i> and <i>Soprano</i> LTR are involved in primordial germ cell development.

iScience·2026
Same author

Switchable photoluminescence of europium(III) complexes with chromonylhydrazones.

Chemical communications (Cambridge, England)·2026
Same author

Lifetime sports and exercise experience are associated with cognitive, social, and resting-state brain activity in adults.

Brain imaging and behavior·2026
Same author

Three-dimensional analysis of maxillary asymmetry in patients with mandibular prognathism.

Maxillofacial plastic and reconstructive surgery·2026
Same author

[Pulmonary Arterial Hypertension Impairs Duodenal Barrier Integrity in Rats].

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·2026
Same author

ATAC-seq of low-input and cryopreserved primordial germ cells reveals functional enhancers.

Development (Cambridge, England)·2026

Related Experiment Video

Updated: Feb 12, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

8.8K

Polyamines protect nucleic acids against depurination.

Yusuke Terui1, Taketo Yoshida1, Akihiko Sakamoto1

  • 1Faculty of Pharmacy, Chiba Institute of Science, Choshi, Chiba, Japan.

The International Journal of Biochemistry & Cell Biology
|April 13, 2018
PubMed
Summary
This summary is machine-generated.

Polyamines, especially longer and branched types, significantly protect DNA and RNA from heat-induced depurination. These molecules are crucial for maintaining nucleic acid stability at high temperatures.

Keywords:
DepurinationPolyamineStandard polyaminesThermus thermophilusUnusual polyamines

More Related Videos

NanoDrop Microvolume Quantitation of Nucleic Acids
09:28

NanoDrop Microvolume Quantitation of Nucleic Acids

Published on: November 22, 2010

207.2K
A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

8.5K

Related Experiment Videos

Last Updated: Feb 12, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

8.8K
NanoDrop Microvolume Quantitation of Nucleic Acids
09:28

NanoDrop Microvolume Quantitation of Nucleic Acids

Published on: November 22, 2010

207.2K
A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

8.5K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Depurination, the loss of purine bases from nucleic acids, is accelerated by heat and oxidative stress.
  • Polyamines were previously shown to mitigate heat shock and oxidative stress by stimulating protective protein synthesis.
  • This study investigates the direct protective role of polyamines against thermal depurination of nucleic acids.

Purpose of the Study:

  • To determine if polyamines directly protect nucleic acids (DNA and RNA) from thermal depurination.
  • To compare the protective efficiencies of different polyamines (standard, longer, branched) and magnesium ions (Mg2+).
  • To elucidate the structural features of polyamines that enhance nucleic acid protection.

Main Methods:

  • Investigating the depurination suppressing efficiencies of various polyamines (spermine, caldopentamine, caldohexamine, mitsubishine, tetrakis(3-aminopropyl)ammonium) on DNA and tRNA.
  • Conducting experiments in the presence of 1 mM Mg2+.
  • Comparing the protective effects of polyamines against Mg2+ alone.

Main Results:

  • Polyamines significantly suppressed nucleic acid depurination, with longer and branched polyamines showing higher efficiency than standard ones.
  • Spermine, caldopentamine, and caldohexamine protected DNA with 50-80% efficiency and tRNA with 60-80% efficiency.
  • Mg2+ also offered protection but to a lesser extent than polyamines; branched polyamines were more effective for RNA than DNA.

Conclusions:

  • Polyamines play a direct and crucial role in protecting nucleic acids from thermal depurination.
  • The length and branching of polyamines are key factors determining their protective efficacy.
  • Longer and branched polyamines are vital for maintaining nucleic acid functional conformation under high-temperature stress.