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Updated: Feb 11, 2026

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart
Published on: September 15, 2018
Biobeam-Multiplexed wave-optical simulations of light-sheet microscopy
Martin Weigert1,2, Kaushikaram Subramanian1,2, Sebastian T Bundschuh1
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Abstract:
Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 105-106 multiplexed and GPU-accelerated point-spread-function calculations. The wave-optical nature of these simulations leads to the faithful reproduction of spatially varying aberrations, diffraction artifacts, geometric image distortions, adaptive optics, and emergent wave-optical phenomena, and renders image-formation in light-sheet microscopy computationally tractable.
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