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Biobeam-Multiplexed wave-optical simulations of light-sheet microscopy
Martin Weigert1,2, Kaushikaram Subramanian1,2, Sebastian T Bundschuh1
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Plos Computational Biology
|April 14, 2018
Summary
Biobeam is a new open-source software for simulating light-sheet microscopy. It accurately models image degradation and optical phenomena, making image formation computationally manageable.
Area of Science:
- Optical microscopy
- Computational imaging
Background:
- Image degradation in light-sheet microscopy is a complex wave-optical challenge.
- Accurate simulation of optical phenomena is crucial for understanding image formation.
Purpose of the Study:
- To introduce biobeam, an open-source software package for simulating light-sheet microscopes.
- To provide a computationally tractable method for modeling wave-optical effects in light-sheet microscopy.
Main Methods:
- Developed biobeam, an open-source software package.
- Integrated multiplexed, GPU-accelerated point-spread-function calculations.
- Employed wave-optical simulations.
Main Results:
- Biobeam enables faithful reproduction of spatially varying aberrations.
- Simulations accurately depict diffraction artifacts and geometric image distortions.
- The software accounts for adaptive optics and emergent wave-optical phenomena.
Conclusions:
- Biobeam offers a powerful tool for simulating operational light-sheet microscopes.
- The software renders image formation computationally tractable.
- Enables accurate prediction and correction of image degradation in light-sheet microscopy.
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