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Updated: Feb 11, 2026

Author Spotlight: Advancing Gene Silencing Research in Silkworms with dsRNA Delivery Through Feeding Chitosan Nanoparticles
Published on: October 4, 2024
Ascorbic acid prevents cellular uptake and improves biocompatibility of chitosan nanoparticles
Hisham A Elshoky1, Taher A Salaheldin2, Maha A Ali3
1Nanotechnology and Advanced Materials Central Lab., Agriculture Research Center, Giza, Egypt; Regional Center for Food and Feed, Agriculture Research Center, Giza, Egypt.
Abstract:
Chitosan nanoparticles have many applications, such as gene and drug delivery, due to their biocompatibility. Chitosan nanoparticles are currently produced by dissolution in acetic acid that affects the biocompatibility at acidic pH. Here, we synthesized and characterized chitosan (CS) and ascorbate chitosan (AsCS) nanoparticles and investigated their cytotoxic effects, internalization, and distribution in the human colon carcinoma cell line using confocal laser scanning microscopy (CLSM). The CS and AsCS nanoparticles were spherical with average particle sizes of 44±8.4nm and 87±13.6nm, respectively. CS nanoparticles were taken up by the cells and showed dose-dependent cytotoxicity. By contrast, AsCS nanoparticles were not internalized and showed no cytotoxicity. Therefore, AsCS nanoparticles are more biocompatible than CS nanoparticles and may be more suitable for extracellular drug delivery.
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