Dwarf chickens with low monocytes/macrophages phagocytic activity show low antibody titers but greater performance

Monocytes/macrophages phagocytosis has key roles in inflammatory responses. However, systematic research on the effects of monocytes/macrophages phagocytosis on production and reproductive performance in dwarf chickens is lacking. In this study, we developed the HCT-8-MTT method to detect monocytes/macrophages phagocytosis product (PP) which was accuracy, flexible, and saving time. Based on PP in 990 dwarf chickens (890 hens and 100 cocks), chickens were divided into high phagocytosis product group (HPPG) and low phagocytosis product group (LPPG). In production performance, chickens in LPPG have higher laying rate at 24 wk and 71 wk and higher average egg weight at 23 wk and 24 wk than in HPPG (P < 0.05). The levels of follicle-stimulating hormone and luteinizing hormone were higher in LPPG than in HPPG at 58 wk (P < 0.01). In the reproductive performance, the fertilization rate in LPPG was higher than that in HPPG at 45 wk, 49 wk, and 53 wk (P < 0.05). Chickens in LPPG have higher hatchability than HPPG at 45 wk and 49 wk (P < 0.05). In LPPG, the mRNA expression levels of follicle-stimulating hormone receptor and CD9 in the follicle were higher than HPPG (P < 0.05). In the immune response, chickens with higher PP levels showed higher antibody titers for the avian influenza virus H9 inactivated vaccine (P < 0.01). Therefore, monocytes/macrophages PP was positively associated with antibody titers and negatively related to production and reproductive performance, and these findings have practical applications for the optimization of production in the poultry industry.