LC-MS Untargeted Protocol for the Analysis of Wine

Area of Science:

• Analytical Chemistry
• Food Science
• Metabolomics

Background:

• Metabolomic fingerprinting is crucial for understanding wine composition.
• Accurate and efficient analytical methods are needed for wine analysis.
• Existing methods may require complex sample preparation.

Purpose of the Study:

• To describe a streamlined protocol for wine metabolomic analysis.
• To validate a method for comprehensive wine fingerprinting.
• To enable applications in oenological research.

Main Methods:

• Liquid chromatography-mass spectrometry (LC-MS) was employed.
• A simplified, single-step dilution sample preparation was developed.
• Orthogonal chromatography (reversed-phase C18 and HILIC) was used for analyte separation.
• High-resolution quadrupole time-of-flight mass spectrometry (HR-QTOF-MS) with dual electrospray ionization (ESI) modes was utilized.

Main Results:

• The protocol allows for straightforward sample preparation.
• Dual-column chromatography provides orthogonal selectivity for comprehensive separation.
• Dual-mode ESI generates four distinct chromatograms per sample.
• The method is validated for wine metabolomic fingerprinting.

Conclusions:

• The described LC-MS protocol is efficient and effective for wine metabolomics.
• This method can be applied to studies on wine quality and authenticity.
• The protocol's simplicity and comprehensiveness make it valuable for oenological research.