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A modified photometric quantification of skin surface lipids sampled using the cigarette paper method.
S Bolanca-Bumber1, A Basta-Juzbasić, A Kansky
1Department of Dermatology, Clinical Center Zagreb, Yugoslavia.
Archives of Dermatological Research
|January 1, 1988
Summary
A new photometric method accurately measures skin surface lipids (SSLs) for monitoring acne treatments. This noninvasive technique is as reliable as traditional methods for assessing sebum levels.
Area of Science:
- Dermatology
- Biochemistry
- Analytical Chemistry
Background:
- Severe acne conglobata often requires treatments that reduce sebum excretion, like 13-cis-retinoic acid.
- Monitoring treatment efficacy necessitates accurate, noninvasive methods for measuring skin surface lipids (SSLs).
Purpose of the Study:
- To introduce and validate a modified photometric quantification method for assessing SSLs.
- To compare the accuracy and reproducibility of the new photometric method against established techniques.
Main Methods:
- Utilized a modified photometric quantification of SSLs.
- Employed the cigarette paper method for lipid sampling.
- SSLs were quantified based on a reaction with phosphovanilic reagent.
- Compared photometric results with gravimetric techniques.
Main Results:
- The photometric method demonstrated accuracy comparable to the gravimetric technique for SSLs assessment.
- The method proved reproducible across a range of low and high SSL levels.
- A 3-hour assessment period was found to be sufficient for accurate photometric SSL measurement.
Conclusions:
- The modified photometric quantification offers a noninvasive and accurate alternative for measuring SSLs.
- This method is suitable for monitoring the effects of sebum-reducing acne treatments.
- The technique provides reliable and reproducible results in diverse lipid level scenarios.