Multiplexed Isobaric Tag-Based Profiling of Seven Murine Tissues Following In Vivo Nicotine Treatment Using a...

Area of Science:

• Proteomics
• Toxicology
• Biochemistry

Background:

• Nicotine, a primary addictive component in tobacco and e-cigarettes, is known to affect cellular pathways.
• The precise mechanisms by which nicotine induces protein alterations across various cell types remain largely undetermined.
• Understanding these mechanisms is crucial for assessing the health impacts of nicotine exposure.

Purpose of the Study:

• To investigate the effects of oral nicotine administration on protein abundance across multiple tissues in a murine model.
• To identify tissue-specific and global protein alterations resulting from nicotine exposure.
• To establish a comprehensive proteomic profile of nicotine's impact on different organs.

Main Methods:

• Proteomic profiling of seven tissues (brain, heart, kidney, liver, lung, pancreas, spleen) from nicotine-treated and control mice.
• Utilized TMT10-plex technology for efficient multiplexed quantification of proteins.
• Employed a streamlined sample preparation protocol involving TMT reagents and centrifugation-based reversed-phase columns.

Main Results:

• Quantified over 11,000 non-redundant proteins and 138,000 peptides across seven tissues.
• Identified significant alterations in protein abundance in tissues from nicotine-exposed mice, ranging from 7 to 126 proteins per tissue.
• Discovered 11 proteins that were significantly altered in two or more tissues, indicating a systemic effect of nicotine.

Conclusions:

• Nicotine exposure induces widespread changes in protein abundance across multiple murine tissues.
• The proteomic data demonstrate a significant systemic impact of nicotine, affecting various organs.
• This study provides foundational proteomic insights into the molecular consequences of nicotine exposure.