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Protein Kinase C-delta Inhibitor Peptide Formulation using Gold Nanoparticles
Published on: March 9, 2019
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Gold nanoparticles functionalized with angiogenin-mimicking peptides modulate cell membrane interactions
Lorena M Cucci1, Alessia Munzone2, Irina Naletova1
1Department of Chemical Sciences, University of Catania, Viale A. Doria 6, 95125 Catania, Italy.
Biointerphases
|April 18, 2018
Summary
Hybrid nanoassemblies of gold nanoparticles and angiogenin fragments were created to study cell membrane interactions. These assemblies show potential for cancer therapy by influencing cell proliferation and migration.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Cell Biology
Background:
- Angiogenin is vital for angiogenesis, overexpressed in cancers, and downregulated in neurodegenerative diseases.
- Angiogenin's interaction with actin (residues 60-68) is key for cytoskeleton reorganization, affecting cell proliferation and migration.
- Understanding these interactions is crucial for developing targeted therapies.
Purpose of the Study:
- To create and characterize hybrid nanoassemblies of gold nanoparticles and angiogenin fragments.
- To investigate the interaction of these nanoassemblies with model and cellular membranes.
- To compare different binding mechanisms and peptide modifications.
Main Methods:
- Preparation and characterization of gold nanoparticle-angiogenin fragment hybrid nanoassemblies.
- Utilizing supported lipid bilayers (SLBs) and cancer/normal cell lines (neuroblastoma, fibroblasts).
- Employing UV-Vis, AFM, CD, cell viability, proliferation, and actin staining assays.
Main Results:
- Hybrid nanoassemblies exhibited stronger membrane interaction than bare nanoparticles.
- Different binding mechanisms (physisorption, chemisorption) and peptide modifications (cysteine, Fam) were elucidated.
- Cell assays revealed slight nanotoxicity in neuroblastoma cells and proliferative activity in fibroblasts.
Conclusions:
- The study successfully developed and characterized novel hybrid nanoassemblies for probing biological interactions.
- These nanoassemblies demonstrate differential effects on cancer and normal cells, suggesting therapeutic potential.
- The findings provide insights into nanoparticle-peptide interactions at the cellular level.
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