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Updated: Feb 11, 2026

A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle
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Identification and Functional Analysis of Long Intergenic Non-coding RNAs Underlying Intramuscular Fat Content in

Cheng Zou1, Long Li1, Xiaofang Cheng1

  • 1Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education and Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China.

Frontiers in Genetics
|April 18, 2018
PubMed
Summary

This study identifies 1,032 long intergenic non-coding RNAs (lincRNAs) in pig muscle, revealing their role in regulating intramuscular fat (IMF) content and pork quality. Six hub lincRNAs may be crucial for IMF development.

Keywords:
co-expression networkintramuscular fat contentlincRNAmethylationpig

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Area of Science:

  • Animal Genomics
  • Molecular Biology
  • Gene Regulation

Background:

  • Intramuscular fat (IMF) is key to pork quality, with its regulation involving numerous genes.
  • Long intergenic non-coding RNAs (lincRNAs) are increasingly recognized as critical regulators in biological processes.
  • The role and mechanisms of lincRNAs in regulating pig IMF remain largely unexplored.

Purpose of the Study:

  • To identify and characterize lincRNAs involved in the development of intramuscular fat in pigs.
  • To elucidate the regulatory mechanisms of lincRNAs in IMF deposition.
  • To identify potential lincRNA targets for molecular-assisted breeding in pigs.

Main Methods:

  • RNA sequencing of pig longissimus dorsi muscle across four stages of varying IMF content.
  • Bioinformatic reconstruction of transcripts and identification of lincRNAs.
  • Analysis of lincRNA expression, methylation patterns, and co-expression networks.

Main Results:

  • 1,032 lincRNAs were identified in pig longissimus dorsi muscle.
  • lincRNAs exhibit distinct characteristics, including shorter length and lower expression compared to protein-coding genes.
  • Both promoter and genebody methylation negatively regulate lincRNA expression.
  • Co-expression analysis revealed stage-specific modules, with some lincRNAs linked to fatty acid metabolism and PPAR signaling.
  • Six hub lincRNAs were identified as potentially significant regulators of IMF development.

Conclusions:

  • This study provides a comprehensive catalog of lincRNAs associated with pig IMF.
  • lincRNAs are implicated in key metabolic pathways influencing IMF.
  • Identified lincRNAs offer potential targets for improving pork quality through molecular breeding strategies.