Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The anionic matrix at the rat glomerular endothelial surface.

P S Avasthi1, V Koshy

  • 1Research Service, V.A. Medical Center, Albuquerque, New Mexico.

The Anatomical Record
|March 1, 1988
PubMed
Summary

A continuous anionic matrix lines glomerular capillaries, composed mainly of sialoproteins on the cell surface and heparan sulfate, hyaluronic acid, and sialoproteins within fenestral channels. Its function remains unknown.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Standardisation of the cattell's infant intelligence scale in India.

Indian journal of psychiatry·2011
Same author

Combating aggression in institutionalized childern with physical exercise and music.

Indian journal of psychiatry·2011
Same author

Exercise training by individuals with predialysis renal failure: cardiorespiratory endurance, hypertension, and renal function.

American journal of kidney diseases : the official journal of the National Kidney Foundation·1997
Same author

Serum albumin in continuous peritoneal dialysis: its predictors and relationship to urea clearance.

Kidney international·1996
Same author

Creatinine clearance in continuous peritoneal dialysis: dialysis dose required for a minimal acceptable level.

Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis·1996
Same author

The minimal dose of dialysis required for a target KT/V in continuous peritoneal dialysis.

Clinical nephrology·1995

Area of Science:

  • Nephrology
  • Cell Biology
  • Biochemistry

Background:

  • The anionic macromolecules on the glomerular endothelial cell surface are crucial for kidney function but require specific staining for visualization.
  • Understanding the arrangement and composition of this anionic matrix is essential for comprehending glomerular filtration dynamics.

Purpose of the Study:

  • To investigate the arrangement and composition of the anionic matrix at the luminal surface of glomerular capillaries.
  • To identify the specific anionic constituents present in this matrix and their distribution.

Main Methods:

  • Perfusion of rat kidneys with different types of ferritin (anionic, neutral, and cationized) to assess binding patterns.
  • In vivo enzymatic digestion (neuraminidase, papain) followed by cationized ferritin binding to identify anionic components.

Related Experiment Videos

  • Microscopic visualization of ferritin binding to map the anionic matrix.

    • Main Results:

    • Cationized ferritin (CF) bound extensively to the cell plasmalemma and fenestral spaces, indicating a continuous anionic layer.
    • Anionic ferritin did not bind, while neutral ferritin showed discontinuous binding to the basement membrane.
    • Neuraminidase digestion showed sialoproteins dominate the plasmalemma's anionic sites, whereas fenestral channels contain heparan sulfate, hyaluronic acid, and sialoproteins.
    • Papain digestion indicated the absence of glycolipids at the luminal surface.

    Conclusions:

    • A continuous anionic matrix lines the entire luminal surface of glomerular capillaries, filling fenestrae and interposing between blood and basement membrane.
    • The matrix composition varies, with sialoproteins prevalent on the cell surface and a mix of glycosaminoglycans and sialoproteins in fenestral channels.
    • The functional significance of this anionic layer in glomerular capillaries requires further investigation.