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Anti-bromodeoxyuridine monoclonal antibody: an alternative tool for the identification of replicated DNA at the
1Laboratoire de Cytologie et Histologie animale, Liege, Belgique.
Biology of the Cell
|January 1, 1988
Abstract:
A new method for identifying the replicated DNA at the electron microscope level is described. Cells were first exposed in vitro to 5-bromodeoxyuridine (BUdR) in conjunction with 5-fluorodeoxyuridine (FUdR) and BUdR incorporated into DNA was then detected on Lowicryl-embedded sections by immunogold technique using a monoclonal anti-BUdR antibody. After using this method, chromatin and chromosomes are strongly labelled.