Encapsulating Viability of Multi-strain Lactobacilli as Potential Probiotic in Pigs

Important aspects of the selection of probiotics to be used for mixing in animal feed include host species specificity and probiotic cell survival during production and storage of their products. The research was to screen and investigate some probiotic properties of lactic acid bacteria (LAB) isolated from pig fecal samples. One hundred and thirty-eight representative LAB isolates, which were isolated from 51 pig fecal samples, were tested for acid and bile tolerance, antimicrobial susceptibility, antibacterial activity, potential adhesion to the cell surface, and survival rates when stored in varied microencapsulation forms: freeze-dried, spray-dried, and micro-beads. The antibacterial activity results of the ten LAB isolates, which were acid- (pH 2, 3 h) and bile- (50% (v/v) fresh pig bile, 8 h) tolerant and suitable for resisting the five antibiotics commonly used for treating pig infections with pathogenic indicator strains, showed that three isolates (L21, L80, L103) had strong inhibition to Escherichia coli, Salmonella group B, and Salmonella group D using co-culturing and agar spot assays. The three isolates had high hydrophobicity (65-73%) and did not show antagonistic growth against each other. All three selected isolates had greater than 80% survival in freeze-dried and micro-bead forms at 25-30 °C after 2 days of storage (80.4-86.75%, 7.31-7.89 log CFU/ml). Sequence analysis of the 16S rRNA genes demonstrated that the three isolates belong to Lactobacillus plantarum (strain L21 and strain L80) and L. paraplantarum (strain L103). The single and multiple strains of these bacteria may have potential use as probiotics in pig diets.