Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Protein Maturation01:26

Bacterial Protein Maturation

588
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
588
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

739
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
739
Bacterial Signaling01:30

Bacterial Signaling

41.3K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
41.3K
Protein-protein Interfaces02:04

Protein-protein Interfaces

14.8K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.8K
Bacterial Transformation01:33

Bacterial Transformation

60.2K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
60.2K
Protein and Protein Structure02:15

Protein and Protein Structure

88.9K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
88.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bacterial cell envelope components of gut commensals: effects in host-microbe interaction.

Annals of medicine·2026
Same author

<b>Self-aggregating</b> <i><b>Lactiplantibacillus plantarum</b></i> <b>enhances type-I interferon responses via the cytosolic sensors NOD2 and cGAS</b>.

Gut microbes·2026
Same author

Mixing and fermenting chickpea and almond milk analogues to produce probiotic yoghurt analogues.

Food research international (Ottawa, Ont.)·2025
Same author

[RESRIP, a healthcare network for pediatric inflammatory diseases].

Soins. Pediatrie, puericulture·2025
Same author

An innovative functional fermented vegetable milk analogue fermented by <i>Acidipropionibacterium acidipropionici</i>, <i>Lactobacillus delbrueckii</i> and <i>Lactococcus lactis</i> mitigates the inflammatory response <i>in vitro</i> and <i>in vivo</i>.

Food & function·2025
Same author

Postbiotic Effect of Escherichia coli CEC15 and Escherichia coli Nissle 1917 on a Murine Model of 5-FU-induced Intestinal Mucositis.

Probiotics and antimicrobial proteins·2024
Same journal

Mapping the multigenomic human system: structural asymmetry and interface gaps in host-exogenous biological interactions.

Frontiers in microbiology·2026
Same journal

Bacterial resistance across habitats: from German schools to the International Space Station.

Frontiers in microbiology·2026
Same journal

Correction: Unlocking plant growth-promoting traits of endophytic actinobacteria isolated from <i>Anacyclus pyrethrum</i>, an endemic medicinal plant of the Aguelmam azegza region, Morocco.

Frontiers in microbiology·2026
Same journal

Research progress on <i>Avibacterium paragallinarum</i> and related bacterial and viral diseases in poultry and their mixed infections.

Frontiers in microbiology·2026
Same journal

Development and validation of a quantitative method for the enumeration of <i>Salmonella enterica</i> serovar Infantis from environmental poultry feces based on most probable number approach followed by confirmatory qPCR.

Frontiers in microbiology·2026
Same journal

Multi-omics insights into the microbial and metabolic drivers of regional flavor diversity in Guizhou traditional fermented fish.

Frontiers in microbiology·2026
See all related articles

Related Experiment Video

Updated: Feb 11, 2026

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

11.6K

Extractable Bacterial Surface Proteins in Probiotic-Host Interaction.

Fillipe L R do Carmo1,2, Houem Rabah2,3, Rodrigo D De Oliveira Carvalho1

  • 1Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.

Frontiers in Microbiology
|April 20, 2018
PubMed
Summary
This summary is machine-generated.

Surface layers, protein coats on Gram-positive bacteria, vary greatly between species. These structures and associated proteins are crucial for probiotic bacteria

Keywords:
adhesionhostimmunomodulationprobioticsurface layer protein

More Related Videos

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
07:42

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

Published on: March 19, 2010

11.2K
Biomimetic Materials to Characterize Bacteria-host Interactions
12:22

Biomimetic Materials to Characterize Bacteria-host Interactions

Published on: November 16, 2015

10.0K

Related Experiment Videos

Last Updated: Feb 11, 2026

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

11.6K
In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
07:42

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

Published on: March 19, 2010

11.2K
Biomimetic Materials to Characterize Bacteria-host Interactions
12:22

Biomimetic Materials to Characterize Bacteria-host Interactions

Published on: November 16, 2015

10.0K

Area of Science:

  • Microbiology
  • Probiotics
  • Bacterial Surface Structures

Background:

  • Gram-positive bacteria, including probiotics, possess an outer proteinaceous coat known as a surface layer.
  • This surface layer is formed by the self-assembly of surface-layer proteins (Slp), creating a paracrystalline structure.
  • While the surface layer is conserved across prokaryotes, the Slp protein sequence exhibits significant variability.

Purpose of the Study:

  • To explore the diverse functions of surface layers and associated proteins (SLAPs) in probiotic Gram-positive bacteria.
  • To understand the role of these structures in host interactions, including stress tolerance and gut survival.
  • To investigate their involvement in adhesion, mucus interaction, and modulation of intestinal inflammation.

Main Methods:

  • Analysis of surface layer protein (Slp) variability across different bacterial species and strains.
  • Extraction and characterization of surface layer associated proteins (SLAPs).
  • Experimental approaches to assess the functional roles of surface layers in host interactions.

Main Results:

  • Surface layer proteins (Slp) show high sequence variability, indicating strain-specific adaptations.
  • Associated proteins (SLAPs) contribute to various functions, including host interaction.
  • Probiotic bacteria utilize surface layers for stress tolerance, survival in the digestive tract, adhesion, and immune modulation.

Conclusions:

  • Surface layers and associated proteins are key determinants of probiotic-host interactions.
  • Their functional roles are diverse and strain-dependent, impacting survival and host health.
  • Future applications include using these structures for nanoparticles, coatings, encapsulation, and vaccine development.