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Making It All Work: Functional Genomics and Reporter Gene Assays
Genevieve Welch1,2, Robert Damoiseaux3,4,5, Loren Miraglia6,7
1Department of Genomics, The Genomics Institute of the Novartis Research Foundation, San Diego, CA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|April 20, 2018
Summary
Functional genomics uses reporter gene assays for high-throughput screening to understand gene function. This approach accelerates scientific discovery through speed and quantitative, genome-wide insights.
Area of Science:
- Genomics
- Molecular Biology
- Biotechnology
Background:
- Functional genomics studies gene function across entire genomes.
- Reporter gene assays are key tools for dissecting biological pathways and identifying drug targets.
- Genome-wide studies require a shift from traditional hypothesis-driven research to high-throughput methodologies.
Purpose of the Study:
- To outline a standard functional genomics project utilizing reporter gene assays.
- To provide an overview of the methodologies involved in high-throughput functional genomics.
- To familiarize readers with data analysis techniques for genome-wide studies.
Main Methods:
- High-throughput experimentation.
- Automation in assay development and execution.
- Rigorous validation of automated processes and assays.
Main Results:
- Enables rapid, quantitative, and genome-wide insights into gene function.
- Facilitates the dissection of signaling cascades and identification of novel drug targets.
- Supports deciphering gene expression functions on a large scale.
Conclusions:
- High-throughput functional genomics accelerates scientific progress.
- Reporter gene assays are versatile tools for genome-wide functional studies.
- The approach offers speed, quantitative data, and broad biological insights.
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