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Air content measurement in concrete is critical for ensuring structural integrity and durability of concrete structures, especially in environments prone to severe weather conditions. Accurate air content analysis optimizes concrete's resistance to freeze-thaw cycles and enhances its workability and strength. Several methods are standardized under ASTM guidelines to measure the air content in fresh concrete, each suitable for different concrete types and conditions.
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Updated: Feb 11, 2026

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
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High-Content Reporter Assays.

Erica Cook1, Jeffrey Hermes2, Jing Li3

  • 1Lead Discovery and Optimization, Bristol Myers Squibb, Pennington, NJ, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 20, 2018
PubMed
Summary
This summary is machine-generated.

High-content screening minimizes artifacts in fluorescent reporter assays, enabling cell-by-cell assessment of HIV reactivation compounds in Jurkat cells.

Keywords:
1536-well platesDrug discoveryHCSHIVHTSHigh-content screeningHigh-throughput screeningLatencyLentivirusReactivationReporter gene assay

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Virology

Background:

  • Fluorescent reporters are challenging for drug screening due to artifacts like compound fluorescence and cellular debris.
  • Traditional whole-well fluorescence assays struggle to accurately assess reporter activation.

Purpose of the Study:

  • To demonstrate the utility of high-content screening (HCS) for overcoming limitations of fluorescent reporters.
  • To develop a method for screening HIV reactivation compounds using fluorescent reporters and HCS.

Main Methods:

  • Utilized high-content screening for individual cell isolation and segmentation.
  • Employed a fluorescent reporter system in Jurkat cells to monitor HIV reactivation.
  • Applied cell-by-cell analysis to mitigate artifacts in fluorescence signal assessment.

Main Results:

  • HCS enabled accurate assessment of fluorescent reporter activation at the single-cell level.
  • Artifacts commonly seen in whole-well assays were effectively minimized.
  • Facilitated reliable screening for compounds inducing HIV reactivation.

Conclusions:

  • High-content screening is a powerful approach for utilizing fluorescent reporters in drug discovery.
  • This method enhances the reliability of screening for modulators of viral reactivation, such as HIV.