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Vitamin B12 (cyanocobalamin) in Infant Formula Adult/Pediatric Nutritional Formula by Liquid Chromatography with

Ester Campos Giménez1, Frédéric Martin1

  • 1Nestlé Research Center, Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland.

Journal of AOAC International
|April 21, 2018
PubMed

Insights

This study validated the AOAC Official MethodSM 2014.02 for determining Vitamin B12 in infant formula. The method demonstrated acceptable repeatability and reproducibility, meeting performance requirements for reliable vitamin B12 analysis.

Area of Science:

  • Analytical Chemistry
  • Food Science
  • Nutritional Analysis

Background:

  • Accurate quantification of Vitamin B12 is crucial for infant and adult nutritional formulas.
  • AOAC Official MethodSM 2014.02 was developed for Vitamin B12 determination using liquid chromatography.
  • Validation of analytical methods ensures reliability and standardization in food analysis.

Purpose of the Study:

  • To assess the repeatability and reproducibility of AOAC Official MethodSM 2014.02.
  • To determine if the method meets the Standard Method Performance Requirement (SMPR® 2011.005) for Vitamin B12.
  • To evaluate the method for potential advancement to Final Action status.

Main Methods:

  • A collaborative study involving 21 laboratories across 13 countries.
  • Two-part analysis: initial duplicate testing followed by blind duplicate analysis of 10 samples for qualified labs.
  • Comparison of results against established Standard Method Performance Requirements (SMPR®).

Main Results:

  • Repeatability relative standard deviation ranged from 1.1% to 6.5%.
  • Reproducibility relative standard deviation ranged from 6.0% to 23.8%, with one matrix exceeding the 11% requirement.
  • Horwitz ratio values were consistently below 2 (0.17-0.78), indicating good method performance.

Conclusions:

  • The AOAC Official MethodSM 2014.02 demonstrated acceptable precision for Vitamin B12 determination in infant and adult formulas.
  • The method met the SMPR® requirements, supporting its reliability.
  • The AOAC Expert Review Panel recommended the method for Final Action status.

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