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YWHAZ Binds to TRIM21 but Is Not Involved in TRIM21-stimulated Osteosarcoma Cell Proliferation
Qing Zhong Zeng1, Wan Ting Liu1, Jun Lei Lu1
1Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, Jinan University, Guangzhou 510632, Guangdong, China.
Biomedical and Environmental Sciences : BES
|April 21, 2018
Summary
Tripartite motif 21 (TRIM21) promotes osteosarcoma cell proliferation and stress tolerance. TRIM21 interacts with and downregulates YWHAZ, clarifying mechanisms in this bone cancer.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Osteosarcoma is a prevalent pediatric bone cancer.
- E3 ligases are crucial in cancer development and progression.
- The specific role of TRIM21 in osteosarcoma remains under investigation.
Purpose of the Study:
- To investigate the function of the E3 ligase tripartite motif 21 (TRIM21) in osteosarcoma cell proliferation.
- To identify novel interacting partners of TRIM21 in osteosarcoma cells.
Main Methods:
- Cell viability assessed using MTT assays.
- TRIM21 interactions identified via co-immunoprecipitation and LC-MS/MS.
- YWHAZ interaction validated using co-immunoprecipitation and bimolecular fluorescence complementation (BIFC).
Main Results:
- TRIM21 positively regulates osteosarcoma cell proliferation and stress tolerance.
- YWHAZ identified as a novel TRIM21-interacting protein.
- TRIM21 negatively regulates YWHAZ expression, dependent on its RING domain.
Conclusions:
- TRIM21 plays a significant role in osteosarcoma pathogenesis.
- The TRIM21-YWHAZ interaction provides insights into osteosarcoma molecular mechanisms.
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