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Widespread Enhancer Activity from Core Promoters.

Alejandra Medina-Rivera1, David Santiago-Algarra2, Denis Puthier2

  • 1Laboratorio Internacional de Investigación sobre el Genoma Humano, Universidad Nacional Autónoma de México, Juriquilla, Mexico.

Trends in Biochemical Sciences
|April 21, 2018
PubMed
Summary
This summary is machine-generated.

Many core promoter regions exhibit enhancer activity, challenging traditional gene regulation models. This finding, supported by advanced techniques, redefines our understanding of genome organization and gene expression control.

Keywords:
CRISPR/Cas9ePromotersenhancergene regulationpromotersreporter assays

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Area of Science:

  • Molecular Biology
  • Genomics
  • Gene Regulation

Background:

  • Gene expression in higher eukaryotes relies on precise temporal and spatial regulation.
  • This regulation involves the interplay between promoters and gene-distal regulatory elements called enhancers.
  • Traditionally, promoters are defined by local gene induction, while enhancers activate gene expression remotely.

Purpose of the Study:

  • To investigate the phenomenon of core promoter regions exhibiting enhancer activity.
  • To assess the prevalence and impact of this dual functionality on gene regulation understanding.

Main Methods:

  • Utilized high-throughput reporter assays.
  • Employed clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-related approaches.

Main Results:

  • Observed that numerous core promoter regions possess enhancer activity.
  • Indicated that this phenomenon is widespread in gene regulation.

Conclusions:

  • The conventional distinction between promoters and enhancers is challenged by shared genomic and epigenomic features.
  • Core promoters displaying enhancer activity significantly impact the global understanding of genome organization and gene expression regulation.