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High sensitivity and label-free oligonucleotides detection using photonic bandgap sensing structures
Ángela Ruiz-Tórtola1, Francisco Prats-Quílez1, Daniel González-Lucas2
1Nanophotonics Technology Center, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain.
Abstract:
A label-free sensor, based on the combination of silicon photonic bandgap (PBG) structures with immobilized molecular beacon (MB) probes, is experimentally developed. Complementary target oligonucleotides are specifically recognized through hybridization with the MB probes on the surface of the sensing structure. This combination of PBG sensing structures and MB probes demonstrates an extremely high sensitivity without the need for complex PCR-based amplification or labelling methods.
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