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Updated: Feb 11, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
Qualitative defects with normal sperm counts in a patient attending infertility clinic
Rachna Goyal1, Mrinalini Kotru1, Aarti Gogia1
1Department of Pathology, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, India.
Background:
Sperm morphology is an important qualitative parameter in semen analysis.
Aim:
To assess qualitative defects in the semen with normal sperm counts. Setting and Design: This was a prospective and descriptive study done in a tertiary care center.
Materials And Methods:
Seventy-five semen samples with normal sperm counts were evaluated. Qualitative defects of sperms on Papanicolaou-stained slides were assessed. The defects were noted as follows: head (tapering head, large head, small head, round head, bifid head), middle piece (bent neck, broad middle piece, excess residual cytoplasm), and tail (coiled tail).
Results:
All cases showed the qualitative defects in spite of the normal counts. The defects found in head - pointed/tapering head (1%-20%), round head (1%-7%), large head (1%-3%) and others (0%-2%); in middle piece - bent neck (1%-8%), excess cytoplasm (1%-3%) or broad middle piece (1%-12%); and in tail - coiled tail (1%-5%).
Conclusions:
Qualitative defects are often seen even in cases with normal sperm counts.
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