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Updated: Feb 11, 2026
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In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
Published on: December 30, 2016
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An optimized method to measure human FOXP3+ regulatory T cells from multiple tissue types using mass cytometry
Nicholas A J Dawson1,2, Avery J Lam2,3, Laura Cook1,2
1Department of Medicine, University of British Columbia Vancouver, BC, Canada.
European Journal of Immunology
|April 21, 2018
Summary
We optimized mass cytometry to detect FOXP3+ regulatory T cells (Tregs). This method offers a powerful alternative to flow cytometry for analyzing Tregs in various biological samples.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- Regulatory T cells (Tregs) are crucial for immune homeostasis.
- Accurate detection of FOXP3, a key Treg marker, is essential for Treg research.
- Existing methods like flow cytometry have limitations in high-dimensional analysis.
Purpose of the Study:
- To optimize and validate a mass cytometry protocol for FOXP3 detection.
- To compare mass cytometry data with conventional flow cytometry.
- To demonstrate the application of the optimized protocol for Treg profiling in diverse biological samples.
Main Methods:
- Mass cytometry optimization for FOXP3 detection.
- Comparative analysis of mass cytometry and flow cytometry data.
- Application of the protocol to whole blood, cord blood, thymus, and synovial fluid samples.
Main Results:
- An optimized mass cytometry method for FOXP3 detection was established.
- Mass cytometry provided comparable or superior data to flow cytometry.
- The protocol successfully profiled antigen-specific Tregs and Tregs from various tissues.
Conclusions:
- Mass cytometry is a robust and versatile technique for FOXP3+ Treg detection.
- This optimized protocol enhances Treg analysis in immunology research.
- The method facilitates comprehensive Treg profiling across different sample types.
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