Application of a reflective microscope objective for multiphoton microscopy

Related Experiment Video

Updated: Feb 11, 2026

Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP
10:03

Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP

Published on: September 23, 2012

14.3K

Mohammad M Kabir1, Aakash M Choubal2, Kimani C Toussaint2

  • 1Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.

Journal of Microscopy
|April 21, 2018
PubMed

Keywords:
Multiphoton microscopyreflective objectivesecond-harmonic generationtwo-photon fluorescence

More Related Videos

Multiphoton Microscopic Observation of Vessels in Mouse Liver Tissue06:33

Multiphoton Microscopic Observation of Vessels in Mouse Liver Tissue

Published on: May 17, 2021

4.4K
A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva06:53

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

Published on: May 17, 2020

6.1K

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
13.5K
Reflection of Waves01:07

Reflection of Waves

When a wave travels from one medium to another, it gets reflected at the boundary of the second medium. A common example of this is when a person yells at a distance from a cliff and hears the echo of their voice. The sound waves (longitudinal waves) traveling in the air are reflected from the bounding cliff. Similarly, flipping one end of a string whose other end is tied to a wall causes a pulse (transverse wave) to travel through the string, which gets reflected upon reaching the wall. In...
4.7K
Velocity of an Object01:18

Velocity of an Object

Understanding how an object moves along a path requires distinguishing between motion over a time span and motion at a precise moment. A useful example is a vehicle traveling along a straight and level path, where its position at any given time is known. The initial step in analyzing this motion is to measure how far the vehicle travels over a fixed time period. This measurement, called average velocity, is computed by dividing the total change in position by the duration over which the change...
208
The Sense of Self: Reflected Self-Appraisal and Social Comparison02:57

The Sense of Self: Reflected Self-Appraisal and Social Comparison

According to Charles Cooley, we base our image on what we think other people see (Cooley 1902). We imagine how we must appear to others, then react to this speculation. We don certain clothes, prepare our hair in a particular manner, wear makeup, use cologne, and the like—all with the notion that our presentation of ourselves is going to affect how others perceive us. We expect a certain reaction, and, if lucky, we get the one we desire and feel good about it. But more than that, Cooley...
56.1K
Reflective Property of Parabolas01:26

Reflective Property of Parabolas

A parabola is a basic type of conic section that results from the intersection of a plane with a double-napped cone in a direction parallel to one of the cone's sides. This U-shaped curve has a distinctive reflective property: all incoming rays parallel to its axis of symmetry are directed toward a single point, known as the focus. This property is widely utilized in optical and communication technologies that require precise signal concentration.In analytic geometry, a parabola is defined as...
289
Potential Due to a Polarized Object01:29

Potential Due to a Polarized Object

A neutral atom consists of a positively charged nucleus surrounded by a negatively charged electron cloud. When placed in an external electric field, the external electric force pulls the electrons and nucleus apart, opposite to the intrinsic attraction between the nucleus and the electrons. The opposing forces balance each other with a slight shift between the center of masses of the nucleus and the electron cloud, resulting in a polarized atom. On the other hand, a few molecules, like water,...
805