Related Experiment Video
Updated: Feb 11, 2026

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
Published on: June 15, 2018
Analytical and Workflow Evaluation of the ARIES Sample-to-Result Molecular Assay for Clostridium difficile
Steven K Lawson1, David Chung1, Shubhagata Das2
1Vanderbilt University Medical Center, Department of Pathology, Microbiology, and Immunology, Molecular Infectious Diseases Laboratory, Nashville, TN, USA.
Abstract:
We evaluated the analytical and workflow characteristics of the ARIES Clostridium difficile assay, a recently developed qPCR-based test for toxigenic C. difficile ARIES was compared to the illumigene C. difficile assay, a commonly employed, loop-mediated amplification technique with similar sample-to-result capabilities. Following illumigene analysis, 122 positive and 164 negative stool specimens were banked for subsequent ARIES testing. The analytical agreement between the platforms was high: 93.4% positive agreement (89.0-97.8%) and 97.5% negative agreement (95.2-99.9%). For discordant specimens, amplification/bidirectional sequencing of tcdA/tcdB demonstrated toxigenic C. difficile in 2/4 illumigene(-)ARIES(+) and 2/8 illumigene(+)ARIES(-) specimens. In a time-motion study, the ARIES assay required less hands-on time than illumigene, but with greater total testing time. Overall, these findings support the ARIES C. difficile Assay as a new option for laboratories in their diagnostic repertoire.
Related Concept Videos
Resultant of a General Distributed Loading
Examples such as load distribution due to wind and load distribution on a bridge illustrate how this concept is used to analyze and design safe, reliable structures under variable loading conditions. Most structures, such as residential buildings, bridges, and towers, are...
Resultant Moment: Scalar Formulation
To determine the resultant moment, the moments caused by all the forces in a system in the x-y plane are considered. Positive moments are typically...
Resultant Moment: Vector Formulation
The resultant moment of a system of forces can be calculated through vector formulation. For example, if we consider...
Development of Analytical Methods
Molecular Orbital Theory II
Analyte Adsorption and Distribution

