Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

1.2K
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
1.2K
Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

1.6K
The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
1.6K
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

2.4K
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
2.4K
Ionization Energy03:12

Ionization Energy

43.5K
The amount of energy required to remove the most loosely bound electron from a gaseous atom in its ground state is called its first ionization energy (IE1). The first ionization energy for an element, X, is the energy required to form a cation with 1+ charge:
43.5K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

1.9K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.9K
Atomic Mass01:52

Atomic Mass

70.5K
Atoms — and the protons, neutrons, and electrons that compose them — are extremely small. For example, a carbon atom weighs less than 2 × 10−23 g. When describing the properties of tiny objects such as atoms, we use appropriately small units of measure, such as the atomic mass unit (amu). The amu was originally defined based on hydrogen, the lightest element, then later in terms of oxygen. Since 1961, it has been defined with regard to the most abundant isotope of carbon, atoms of which...
70.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stable isotope labeling LC-MS for profiling the amine submetabolome in a D-lactate treated cell model.

Analytica chimica acta·2026
Same author

Catalytic Enantioselective S<sub>N</sub>2'-Type Reaction for Axial Allene Chirality.

Journal of the American Chemical Society·2026
Same author

[Application of percutaneous endoscopic gastrostomy in children with methylmalonic acidemia or propionic acidemia complicated by feeding difficulties: a case series of 7 patients].

Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics·2026
Same author

Gas-Phase F-Atom Migration Reactions of Perfluoroalkyl and Polyfluoroalkyl Sulfonic/Sulfinic Anions.

Rapid communications in mass spectrometry : RCM·2026
Same author

[Mechanism and characteristics of Epimedii Folium and its compound preparations in treatment of depression].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·2026
Same author

Research Progress on Signal Transduction Pathways Related to Breast Cancer and Chinese Medicine Intervention.

Chinese journal of integrative medicine·2025
Same journal

Strategy for Simultaneous Multiomic Survey of N-Glycomic and Extracellular Matrix Proteome by Mass Spectrometry Imaging.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Going a Layer Deeper: Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) to Analyze Degrading Soft Tissue Layers in Post-Mortem Interval (PMI) Analysis.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Slice Collision-Induced Unfolding and Molecular Dynamics Reveal How Post-Translational Succination Reshapes SUMO1 Unfolding Pathways.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Role of Ion Charge and Size in Susceptibility to Acoustic Ion Manipulation (AIM).

Journal of the American Society for Mass Spectrometry·2026
Same journal

Manipulation of Electrospray-Produced Biomolecular Ions with Acoustic Fields at Atmospheric Pressure.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Structural Elucidation of Fc- and Fab-Associated <i>N</i>-Glycans in Cetuximab Using Protein A-Assisted Domain-Resolved Glycan Profiling Using Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
See all related articles

Related Experiment Video

Updated: Feb 11, 2026

Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry
07:53

Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry

Published on: March 1, 2020

7.8K

Fast Eruption Desorption Ionization for Mass Spectrometric Analysis.

Xiao-Pan Liu1, Hao-Yang Wang2, Guo-Qing Dong1

  • 1State Key Laboratory of Organometallic Chemistry and National Center for Organic Mass Spectrometry in Shanghai, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Road, Shanghai, 20032, China.

Journal of the American Society for Mass Spectrometry
|April 22, 2018
PubMed
Summary
This summary is machine-generated.

A new Fast Eruption Desorption Ionization (FEDI) technique offers simple, rapid, and sensitive analysis. It features three modes optimized for volatile, nonvolatile, and fragile compounds, yielding high-quality mass spectra.

Keywords:
EM-assisted eruptionFast eruption desorption ionizationMass spectrometryNon-assisted eruptionSolvent-assisted eruption

More Related Videos

Mass Spectrometric Analysis of Glycosphingolipid Antigens
13:09

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

17.0K
Matrix-assisted Laser Desorption/Ionization Time of Flight MALDI-TOF Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
07:49

Matrix-assisted Laser Desorption/Ionization Time of Flight MALDI-TOF Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

Published on: September 9, 2013

81.8K

Related Experiment Videos

Last Updated: Feb 11, 2026

Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry
07:53

Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry

Published on: March 1, 2020

7.8K
Mass Spectrometric Analysis of Glycosphingolipid Antigens
13:09

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

17.0K
Matrix-assisted Laser Desorption/Ionization Time of Flight MALDI-TOF Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
07:49

Matrix-assisted Laser Desorption/Ionization Time of Flight MALDI-TOF Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

Published on: September 9, 2013

81.8K

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Chemical Analysis

Background:

  • Developing rapid and sensitive analytical techniques is crucial for diverse chemical compound analysis.
  • Existing methods may have limitations in handling various compound types, such as volatile, nonvolatile, or fragile molecules.

Purpose of the Study:

  • To introduce and characterize the Fast Eruption Desorption Ionization (FEDI) technique.
  • To demonstrate the versatility of FEDI across different analytical modes for various compound classes.

Main Methods:

  • Development of the Fast Eruption Desorption Ionization (FEDI) technique.
  • Implementation of three distinct analytical modes: non-assisted, energetic material (EM)-assisted, and solvent-assisted eruption.
  • Analysis of diverse compounds including stable, volatile, nonvolatile (especially metal compounds), and fragile molecules.

Main Results:

  • FEDI enables simple, rapid, and sensitive analysis of various compounds.
  • Non-assisted eruption is effective for stable and volatile compounds.
  • EM-assisted eruption is suitable for nonvolatile molecules, particularly metal compounds.
  • Solvent-assisted eruption is optimized for fragile molecules.
  • High-quality mass spectra with intact ions were obtained in both positive and negative ion modes.

Conclusions:

  • The FEDI technique provides a versatile platform for mass spectrometry.
  • Its three distinct modes allow for tailored analysis of a wide range of chemical compounds.
  • FEDI offers a valuable advancement in rapid and sensitive chemical analysis.