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Transcriptomic Analysis of Xylan Oligosaccharide Utilization Systems in Pediococcus acidilactici Strain BCC-1
Zhao Lei1, Yuqin Wu1, Wei Nie1
1State Key Laboratory of Animal Nutrition, College of Animal Science and Technology , China Agricultural University , 2 Yuanmingyuan West Road , Beijing 100193 , PR China.
Journal of Agricultural and Food Chemistry
|April 24, 2018
Summary
Xylan oligosaccharides (XOS) significantly enhance Pediococcus acidilactici fermentation compared to other sugars. This study reveals XOS promotes sugar absorption and utilization in this probiotic strain.
Area of Science:
- Microbiology
- Biochemistry
- Genomics
Background:
- Xylan oligosaccharides (XOS) are derived from xylan and are recognized for their potential prebiotic properties.
- Pediococcus acidilactici is a probiotic bacterium with potential applications in animal feed.
- Understanding the specific effects of different carbohydrates on bacterial growth is crucial for optimizing probiotic functions.
Purpose of the Study:
- To evaluate the comparative effects of XOS, fructo oligosaccharides (FOS), glucose, and xylose on the proliferation of Pediococcus acidilactici BCC-1.
- To elucidate the molecular mechanisms underlying the prebiotic activity of XOS in Pediococcus acidilactici BCC-1 through transcriptomic analysis.
- To identify genes and pathways involved in XOS utilization by Pediococcus acidilactici BCC-1.
Main Methods:
- De novo sequencing of Pediococcus acidilactici BCC-1 genome.
- Comparative growth experiments using various carbohydrate sources (XOS, FOS, glucose, xylose) and a control.
- Transcriptomic analysis (RNA sequencing) to compare gene expression profiles after XOS and xylose treatments.
Main Results:
- XOS demonstrated superior performance in promoting Pediococcus acidilactici BCC-1 fermentation compared to FOS, glucose, and xylose.
- The genome analysis identified key enzymes and transporters (e.g., ABC transporters, xylanase, xylosidase) involved in XOS metabolism.
- Transcriptome data revealed that XOS significantly upregulated genes related to carbohydrate metabolism, sugar transport, and energy production (TCA cycle) compared to xylose.
Conclusions:
- XOS effectively promotes the growth and fermentation of Pediococcus acidilactici BCC-1.
- The study provides insights into the genetic basis and metabolic pathways for XOS utilization in this bacterium.
- These findings support the potential of XOS as a functional ingredient to enhance the probiotic efficacy of Pediococcus acidilactici BCC-1 in feed applications.
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