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Published on: January 3, 2020
The grIA Mutation in Norfloxacin-Resistant First-Step Mutants and Clinical Isolates of Staphylococcus aureus
Hideyuki Fukuda1, Satoshi Hori2, Keiichi Hiramatsu2
1Central Research Laboratories, Kyorin Pharmaceutical Co Ltd, Tochigi, Japan; Department of Bacteriology, Juntendo University, Tokyo, Japan.
Abstract:
Ofloxacinresistance in Staphylococcus aureus is achieved through 2 sequential events of genetic alteration. The second-step mutation has been identified as that of DNA gyrase, but the first-step mutation for norfloxacin resistance and low-level ofloxacin resistance has not yet been identified. In this paper, we report that single point mutations of grIA, which encodes for the A subunit of topoisomerase IV (GrIA), are found in all of the norfloxacin-resistant first-step in vitro mutants of S. aureus as well as in quinolone-resistant clinical S. aureus strains. The amino acid substitution of the GrIA was Ser-80 (TCC) to Tyr (TAC) or the (TTC), Glu-84 (GAA) to Lys (AAA), or Ala-116 (GCA) to Glu (GAA). The GCA to GAA mutation at codon 116 is a novel mutation, and may be responsible for higher quinoloneresistance than with the other grIA mutations.
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