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Updated: Feb 11, 2026
07:49
Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
Published on: June 8, 2020
8.8K
Tilted Light Sheet Microscopy with 3D Point Spread Functions for Single-Molecule Super-Resolution Imaging in
Anna-Karin Gustavsson1,2, Petar N Petrov1, Maurice Y Lee1,3
1Dept. of Chemistry, Stanford University, 375 North-South Axis, Stanford, CA, USA 94305-4401.
Summary
We developed tilted light sheet microscopy with 3D point spread functions (TILT3D) for high-resolution, 3D super-resolution imaging in thick cells. This flexible platform achieves tens of nanometers precision for subcellular nanostructure visualization.
Area of Science:
- Biophysics
- Microscopy
- Cell Biology
Background:
- 3D super-resolution imaging is crucial for visualizing subcellular nanostructures.
- Existing methods face challenges in achieving high resolution and precision in thick samples.
Purpose of the Study:
- To present TILT3D, a novel imaging platform for 3D super-resolution microscopy.
- To enable low-background, high-precision imaging of nanostructures within thick cells.
Main Methods:
- Tilted light sheet illumination strategy combined with engineered long axial range point spread functions (PSFs).
- Utilizes a standard inverted microscope with minimal custom components.
- Axial position encoding via PSF shape, enabling simple light sheet optics.
Main Results:
- Demonstrated flexible and user-friendly 3D super-resolution imaging in thick mammalian cells.
- Achieved tens of nanometers localization precision for single molecules.
- Successfully imaged mitochondria and the nuclear lamina using double-helix and Tetrapod PSFs.
Conclusions:
- TILT3D provides a powerful tool for 3D super-resolution imaging of cellular structures.
- The platform facilitates live whole-cell single-particle and single-molecule tracking.
- Enables detailed visualization of nanostructures in thick biological samples.
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