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Functional groups are groups of atoms with specific chemical properties that occur within organic molecules and are sometimes denoted as “R”. Functional groups can “functionalize” a compound by enabling it to adopt different physical and chemical properties.
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Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
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Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
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Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
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Advances in Nutritional Metabolomics.

Elizabeth P Ryan1, Adam L Heuberger2, Corey D Broeckling2

  • 1Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins CO 80523.

Current Metabolomics
|April 24, 2018
PubMed
Summary
This summary is machine-generated.

Metabolomics analysis reveals how diet impacts the body's metabolism. This review explores how metabolite profiles in various samples connect dietary changes to health outcomes, aiding nutrition science research.

Keywords:
Nutritionanalytical platformsdietary interventionmetabolismmetabolomics

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Area of Science:

  • Nutrition Science
  • Systems Biology
  • Metabolomics

Background:

  • Metabolomics is an emerging tool in nutrition science for understanding diet's systemic effects.
  • Metabolite profiles from biological samples (tissue, serum, urine, stool) reflect metabolic pathway changes due to diet.

Purpose of the Study:

  • To review the scientific literature connecting individual metabolic profiles with dietary intake variations.
  • To present dietary-metabolomic strategies and their applications in nutrition research.

Main Methods:

  • Review of scientific literature on dietary interventions and metabolomics.
  • Analysis of metabolite variations within and between dietary exposures.
  • Characterization of metabolic responses over time and individual variations.

Main Results:

  • Dietary interventions significantly alter a broad range of metabolites.
  • Metabolite profiles provide insights into metabolic pathway responses to diet.
  • Different metabolite analysis platforms have distinct advantages and drawbacks.

Conclusions:

  • Metabolomics is crucial for understanding nutrition in a systems biology context.
  • Combining targeted and non-targeted metabolomics in future trials will enhance understanding of dietary impacts.
  • Further research integrating metabolomics into dietary studies is warranted.